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文献和实验UPDATED DIRECTIONS FOR NEW BRUNSWICK MOBILE PILOT PLANT FERMENTOR
temperature if not done in step 3 above. 5. Start sterilization cycle. 6. At ~100o open steam and steam drain lines to ports (2 each; acid, base, antifoam, inoculation). Leave open about 2 minutes, then close. Open drain valve steam, vessel drain
to seal and allow to cool before use Eleven liters 2xYT in 4- 4 L flasks, 45 min exposure Eight 500 ml bottles Tubing for harvesting 10% antifoam 289, final concentration in media: 0.01% Start o/n of AMA
31200-80 Stopcock for disposable manual synthesis reaction vessel Luer Lock ring Cole Parmer 45503-19 ?” fitting
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