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文献和实验E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
实验步骤 Harvesting of Bacterial Cells: IMPORTANT: DO NOT EXCEED MAXIMUM CULTURE VOLUMES! High-copy-number maximum culture volume = 15 ml 1. Harvest the bacterial cells: Pellet 10-15ml overnight culture
E.Z.N.A.® HP Total RNA Kit Spin Protocol Eukaryotic Cells and Tissues
to the column. With the spin column inside a 2 ml collection tube (supplied with kit), centrifuge at 10,000 x g for 30-60 seconds min at room temperature. Discard flow-through and proceed to step 6. 6. Place column in a new 2 ml collection tube and add 300
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
is precipitated by adding Protein Precipitation Reagent (PPR). After removal of the protein, the supernatant is mixed with 1 volume of isopropanol to precipitate the RNA. After the centrifugation to pellet the RNA, the supernatant is transfer to a new tube
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