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文献和实验Lipoprotein Isolation - First week
through segments subcutaneously. Do not injure the gut! . 4. Flush hemolymph (yellowish due to the presence of carotin) carefully out by squeezing the thorax area gently. Collect blood from 10-12 locusts. 5. Put the bled locusts in a jar
Lipoprotein Isolation - First week
needle and a capillary tubing (see picture ). 10. Let the hemolymph flow into the heat sealable centrifuge tube. 11. Add another 20 ml PBS into the syringe and overlay on the hemolymph. 12. Turn on the heat sealer to warm it up. 13
Construction and Characterization of Adenovirus Vectors
so that it is parallel to the band and slowly aspirate the band, lowering the needle as the band lowers. Virus from both step gradient tubes can be combined in a single Quick-Seal ultracentrifuge tube. See Troubleshooting. 41
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