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上海希言科学仪器有限公司
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文献和实验Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction
to separate the phases (1,000 X g), and save the aqueous phase (upper phase). 20. Add 2 volumes of 100% Ethanol to each tube and incubate on ice for 5 min. 21. Centrifuge 5 min to collect the precipitate (approximately 5,000 X g). 22. Discard
[Steps 1-22] or SSCP [Steps 23-37] only) Thermal cycler Thin transparent plastic wrap (e.g., Saran Wrap) (for hot-stop PCR [Steps 1-22] or SSCP [Steps 23-37] only) Vertical gel electrophoresis tank for polyacrylamide gels with 21.7 x 16.5-cm glass
of 0.75 to 1. To fix, 1/10 volume of 37% formaldehyde is added to the culture, which is shaken for a further 40 minutes at room temperature. Cells are then recovered by centrifugation at 1400 X g for 2 minutes, and washed (i.e. gently resuspended
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