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文献和实验Cell Thawing/Cell Freezing Protocol
to 37C water bath While holding the tip of the vial, gently agitate the vial, being careful hnot to allow water to penetrate the cap or seal When completely thawed, transfer contents of vial to 15mL test tube Slowly add 10mL warm
Maintenance of Probes in bacteria including Escherichia coli
agar. Use airtight, autoclaved vials containing 2/3 full (appr. 1 ml) of medium. Inoculate each vial with a single colony. Leave the cap of the vial sightly loose and incubate overnight at 37 C.
Maintenance of Probes in bacteria including Escherichia coli
colony. Leave the cap of the vial sightly loose and incubate overnight at 37 C. Seal the vial tightly and stored at room temperature in a dark place. To revive
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