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文献和实验Lipid analysis Week 3: GAS LIQUID CHROMATOGRAPHY
injection mode; with a split ratio of 1:20, only 5 % of the volume injected actually enters the column, while 95 % are diverted. For the exact quantitation with the GLC profile and separate standards, it would be necessary to known what proportion
Lipid analysis－Week 3: GAS LIQUID CHROMATOGRAPHY
of the signals depends of course on the amount injected onto the column. In capillary GLC, only very small volumes can be carried through the column. Since it is impractical to inject 1/20 of a microliter, for example, our instrument uses a split injection mode
where it undergoes “flash methylation” in the hot injection port. Selective ion monitoring and relative retention times are used for the identification and quantitation of barbiturates.
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