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文献和实验Blunt end cloning of PCR produc
% ethanol, dry and resuspend in 20 ul T4 PNK, 20 pmol ATP and 5-10 units T4 PNK. Incubate at 37 C for 1 hour. Ligation reaction: Blunt end ligation is carried out in the presence of 5% PEG 8000. A microgram of Bluescript SK+ is blunt ended (cut
内切酶列表：Enzymes Generating Blunt Ends
Asymmetric sequences are indicated by *. Single letter code: R = G or A; Y = C or T; W = A or T; M = A or C; K = G or T; S = C or G; H = A, C or T; V = A, C or G; B = C, G or T; D = A, G or T;
Blunt end cloning of PCR products
% ethanol, dry and resuspend in 20 ul T4 PNK, 20 pmol ATP and 5-10 units T4 PNK. Incubate at 37 C for 1 hour. Ligation reaction: Blunt end ligation is carried out in the presence of 5% PEG 8000. A microgram of Bluescript SK+ is blunt ended (cut
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