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上海希言科学仪器有限公司
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文献和实验PCR purification kit) purify DNA through Qiaquick column elute DNA into 50 µl H2 O use 0.5 - 1 µl per 25 µl PCR reaction: 1 x 95 °C, 2 min 21 x 95 °C, 30 sec; 60 °C, 30 sec; 72 °C, 1 min 1 x 72
variations) prepare mastermix for all PCR reactions which contain the same T12 MN primer, aliquot 18 µl into each tube and then add the arbitrary primer (= decamer) mix for each 20 µl PCR reaction: 9.2 µl H2 O, 2 µl 10 x PCR reaction buffer
Two dimensional peptide mapping
from the protease adsorbing onto the filter. 3. Aspirate the liquid. Wash the membrane extensively with H2O (5 X 1ml). Then wash with freshly-made 0.05 M NH4HCO3 once or twice. 4. Digestion: Add 150 -200 microliter fresh 0.05 M NH4HCO3
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