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文献和实验Western Blotting with Horseradish Pe
). 2.Aspirate excess PBS. 3.Add 1ml boiling 2X concentrated electrophoresis sample buffer (125mM Tris pH 6.8, 4% SDS, 10% glycerol, 0.006% bromophenol blue, 1.8% beta-mercaptoethanol). 4.Scrape cells from dish, transfer to a microcentrifuge tube, and boil
g. Wash & Lysis: PE: 10 mM PIPES, 1mM EDTA, 8 mM BME Make a 50X stock and pH to 7.2 with KOH LB: 1mM Tris-HCl, 8 mM BME Make Tris as 2M stock and pH to 8.0 with HCl LB + 0.5% NP-40 : (warm for 30' to 37 deg.C
tubes (cold) Solutions: A lot of these solutions are w/w; Tim says that to make these weigh out sucrose and then add buffer till weight is 100g. Wash & Lysis: PE: 10 mM PIPES, 1mM EDTA, 8 mM BME Make a 50X stock
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