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文献和实验【交流】染色体免疫沉淀技术详细步骤-young lab !!!
would have on the results. 1. Add 100 µl Dynal magnetic beads to microfuge tube. Add 1 ml block solution. Set up 1 tube per IP. 2. Collect the beads using magnetic stand. Remove supernatant. 3. Wash beads in 1.5 ml block solution two more times. 4. Resuspend beads
_ChIP_method.html Chromatin IP Method, ChIP buffers, Notes on ChIP Method, Quantative PCR using 32P dCTP. Hahn Lab. ChIP Assay Protocol PDF - /attach/2008/2008-02-01/2B/2B6C4EA25F257424710A50E83CB2F380.pdf Formaldehyde cross-linking
Metabolic labeling Immunoprecipitation
)in dimethylformamide • IP buffer: 1% Triton x-100,0.05% NP-40 in TBS • x2 IP buffer: 2% Triton x-100,0.1% NP-40 in TBS • ProteinG agarose (GIBCO #15920-010) • Gel fixation solution: 7% acetic acid,25% MeOH in water • Gel soaking solution: 1% glycerol,5% PEG
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