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文献和实验PURIFICATION OF PCR PRODUCTS WITH SEPHAD
microtiter plate (Evergreen from Phenix, catalog #LMP-8001. Secure with rubber bands and spin them down at 750x g for 90 sec. Remove the microtiter plate (these can be re-used until they break) and replace it with a new, labeled MJ Research
PURIFICATION OF PCR PRODUCTS WITH SEPHADEX
of parafilm. Incubate for 4 hours at room temperature. After 4 hours, place it on top of a 96-well plate 500ul microtiter plate (Evergreen from Phenix, catalog #LMP-8001. Secure with rubber bands and spin them down at 750x g for 90 sec
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
Need 1.5-2 x 107 cells from a 2 day culture. 1. Cells are harvested as normal, washed x 1 in PBS then taken up at conc. of 1.2 x 10 7 cells/ml in cold PBS. 2. Take x2 Biorad curvettes A. 0.9 ml (107 cells) B. 0.167 ml cells (2 x 106 cells
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