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文献和实验Gene Knockout Techniques(1) The Knockout Process
to the same height as the window of the UV dosimeter on a stable support. We use a styrofoam tube rack for this - it helps to draw an outline of the tissue culture plate on the rack and a circle exactly where the well with worms will be, so you can put the worm
Diamond scribe for writing on slides Pyrex baking dish (~ 24x34x5 cm) UV transparent plastic wrap (e.g. Glad Cling Wrap) 30 slide rack (stainless steel) #113 and 30 slide glass tank, #122 (Shandon Lipshaw, Pittsburgh, PA) 1L glass tank 1L glass
with a license for target validation. A typical 0.2 µmol-scale RNA synthesis provides about 1 milligram of RNA, which is sufficient for 1000 transfection experiments using a 24-well tissue culture plate format. It should be noted that the use of siRNAs
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