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文献和实验Casting Immobilized pH Gradients (IPGs)
conditions. The covalent nature of the chemical bonds formed during the polymerization step results in a permanent stability of the pH gradient within the matrix. Conflicting requirements during the focusing procedure prevent any effective use of IPGs
Capillary Coatings: Choices for Capillary Electrophoresis of DNA
to the silicon atoms. Different authors have reported an overall pKa value of 5.3 ( 1 ) or 6.3 ( 2 ) for surface silanols. In practice, when the capillary surface is bathed in any buffer solution with a pK higher than 2, immobilized negative charges are formed
Fluorescein labeling of proteins
This method uses carboxyfluorescein succinimidyl ester (CFSE)rather than fluorescein isothiocyanate,resulting in more reliable labeling.Succinimidyl esters are excellent reagents for amine modification since the amide products formed are very stable
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