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文献和实验Assaying Cell Cycle Checkpoints: Activity of the Protein Kinase Chk1
in response to DNA damage. Phosphorylation depends on the activity of conserved components of the checkpoint pathway including Rad3, a member of the ATM/ ATR family of kinases. Phosphorylation leads to activation of Chk1 kinase activity. In this chapter
Methods to Study Cancer Therapeutic Drugs That Target Cell Cycle Checkpoints
are activated in response to diverse DNA-damaging agents and radiation, thus representing a critical barrier limiting therapeutic efficacy. To date, despite efforts to target other components of checkpoint signaling pathways (e.g., ATM, Chk2, Wee1), checkpoint
Probing the Telomere Damage Response
Telomere dysfunctions, rendered through replicative attrition of telomeric DNA or due to the inhibition of shelterin components, are recognized as DNA double-stranded breaks (DSBs) by the DNA damage repair (DDR) pathway. This leads
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