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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
1.5 ml 1.2 mM EDTA 0.5M stock 120 µl 1.1% Triton X-100 10% stock 5.5 ml 0.01% SDS 10% stock 50 µl
Transformation of E. coli by Electroporation
ionic strength and a high resistance. The DNA may be purified by either precipitation or dialysis. Purifying DNA by Precipitation: 1. Add 5 to 10 μg of tRNA to a 20 μl ligation reaction. Add 22 μl 5M ammonium acetate. Mix well. 2. Add 100 μl
Cell Culture Media and Solutions
sterilize through a 0.22 � filter and store up to 2 weeks at 4 degrees C. Growth media: (1 liter) 165.0 ml fetal bovine serum, heat inactivated at 50-60 degrees C for one half hour 12.0 ml 200mM (100 X) L-glutamine 1.2 ml 50 mg/ml
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