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文献和实验in the 4℃ walk-in. 16.Transfer the liquid t centrifuge bttles and spin dwn lysate at 8000g fr 1 hur. 17.Decant lysate in a clean beaker.Discard pellet. 18.Filter lysate twice,using Buckner Funnel and Whatman paper as fllws: Cut the Whatman paper t cver
Installing and Configuring CMap
and a map set. View Image Figure 9.8.18 After selecting a map set, the user can select
Porcine brain tubulin preparation
with the equivalent of a Beckman 50.2Ti rotor At least 24 31.5 ml thick-walled polycarbonate ultracentrifuge tubes with screw caps 37℃ water bath 30 ml Dounce "A" glass homogenizer 2L scintered glass filter funnel and 2L sidearm Erlenmeyer
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