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文献和实验Endurance Training Protocol and Longitudinal Performance Assays for Drosophila melanogaster
, which can be pushed down to immobilize the flies while on the Power Tower. Sponge is used for immobilization because its adaptive size allows easy placement near the bottom of the vial. The softer sponge material also reduces injuries caused by the more solid Flugs
-rat IL-6RBiotin1 vial浓缩洗涤液(100X)(Washing Concentrate)5ml标准品(Standards)1 vial显色液(TMB Substrate)6ml标准品稀释液(Standard Diluent)7ml终止液(Stop Solution)5ml浓缩酶联物(HRP)1 vial酶联物稀释液(HRP Diluent)7ml生物素稀释液(Biotin Diluent)7ml四、需要但未提供的材料1、 可在450nm处进行读数的酶标仪;2、 能精确吸取10
. Make a DIG-dNTP mix for PCR as follows: DIG NUCLEOTIDE MIX CONCENTRATIONS Dig-11-dUTP 350 uM dTTP 650 uM dATP 1 mM dCTP 1 mM dGTP 1 mM For each 50 ul of probe synthesized, a 1/10 dilution is made of the DIG-nucleotide mix when added
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