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文献和实验Fastfilter Plasmid Midi Kit Spin Protocol
2. Pellet up to 30-50 ml bacteria in appropriate vessels by centrifugation at 3,500-5,000 x g for 10 min at room temperature. 3. Decant or aspirate medium and discard. To ensure that all traces of the medium are removed, use a clean paper towel to blot
Native Chromatin Preparation and Illumina/Solexa Library Construction
cell isolation kit) per 107 cells. 12. Mix well and incubate for 15 min at 4°C-8°C. 13. Wash cells with T cell isolation buffer by adding 10X-20X the final labeling volume. Centrifuge at 300g for 10 min. Pipette
of EPC medium x 6 hr if a confluent cell layer is desired at the initiation of flow (Fig. 11A). 2) In order to test cell spreading and adhesion under fluid shear stress, allow 3 x 106 cells to adhere for only 15 min before commencement
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