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文献和实验Large Scale Preps: (See Large scale plsasmid prep protocol for more details) Cultures: Inoculate a 5 mL LB/Amp (50 - 100 µg/mL) culture in early a.m. with a single colony. Use all 5 mL to inoculate a 500 mL LB/Amp culture
Two-hybrid analysis of genetic
X-Gal Replica plater and sterile velvets for 150 mm diameter plates.(A replica devise can be fashioned from a box of 200 µl pipet tips by stretching a velvet over the top of the box) 96-prong device (e.g.DanKar MC-96)with 3 mm diameter flat ended metal
Gene Knockout Techniques(1) The Knockout Process
50 (three spots of worm suspension per plate).Synchronize Day 3 (Friday) Synchronize population by egg prep, following the procedure in Barstead, R.J. 1999. Reverse Genetics in ?C. elegans. A Practical Approach.? I.A. Hope, editor, Oxford University Press, NY
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