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上海希言科学仪器有限公司
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文献和实验The ribonuclease protection assay (RPA)
for 5 to 10 minutes (do not dry in a vacuum evaporator centrifuge). Add 50 µl of hybridization buffer and solubilize the pellet by gently vortexing for 20 seconds and quick spin on microfuge. 8. Quantitate duplicate 1 µl samples
100 µl of ice cold 90% ethanol to the pellet. Spin in a microfuge for 5 minutes at 4℃. 7.Carefully remove the supernatant and air dry the pellets for 5 to 10 minutes (do not dry in a vacuum evaporator centrifuge). Add 50 µl of hybridization buffer
Carbohydrate‐Oligonucleotide Conjugates
4,4′‐Dimethoxytrityl chloride Sulfuric acid stain solution [10% (v/v) sulfuric acid in ethanol]
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