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文献和实验Mag-Bind Blood DNA Maximum Yield Protocol (7ml-10ml)
实验试剂 1. Absolute ethanol (96%-100%) 实验设备 1. Nuclease-free 50 ml centrifuge tube 2. Water bath, incubator or heating block preset at 65°C 3. Magnetic separation device for 50 ml tubes
SQ Blood DNA Maxi Protocol for 4-10 ml whole blood
15 ml microcentrifuge tubes 3. Water baths preset at 37°C 4. Paper towels 实验步骤 1. Add one volume of whole blood (or bone marrow) to a nuclease-free 50 ml centrifuge tube containing 3 volume of Buffer ERL. Mix
Preparation of total RNA from whole blood collected in PAXgene tubes
yields of total RNA.use 95% ethanol; 100% ethanol often contains benzene (bad for arrays).8.Apply 700 µl sample to the PAXgene column sitting in a 2 ml processing tube, and centrifuge for 1 min at ³ 8000 x g ( ³ 10,000 rpm). Place the PAXgene column
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