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文献和实验DIRECT AND SHORT-TERM PROCEDURE FOR HARVESTING BONE MARROW CHROMOSOMES
and incubate at 37 C. The A culture is incubated for 30 minutes, the B culture is incubated for 60 minutes. Centrifuge for 6 minutes at 150 x g. For A culture go to Harvest Procedure step G. For B culture go to Harvest Procedure step D. Short-term: Pipet
Endothelial Cell Systems - Instructions For Use
fresh, warm medium to the culture vessel and return to an incubator until fresh trypsinization reagents are available. Quickly transfer the detached cells to a sterile 15 ml centrifuge tube. Rinse the flask with a final 2 ml of HEPES-BSS
Construction and Characterization of Adenovirus Vectors
medium from 150-mm dishes of 293 or A549 cells. ii. Rinse the monolayer twice with 5 mL of 1X citric saline (for 293 cells) or 2 mL of 1X trypsin-EDTA (for A549 cells). iii. For 293 cells, remove
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