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文献和实验Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC
Assays A. Motility B. Dynamics III. Fluorescence Assays A. Oxygen Scavenging B. Assays Back to protocols I. Solutions & Supplies BRB80 (1X): 80 mM PIPES, 1 mM MgCl2 , 1 mM EGTA, pH 6.8 with KOH (generally made as a 5X
Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC
I. Solutions & Supplies BRB80 (1X): 80 mM PIPES, 1 mM MgCl2 , 1 mM EGTA, pH 6.8 with KOH (generally made as a 5X stock and stored at 4¡C) 5 mg/ml casein (Sigma C-5890; dissolve at 10-20 mg/ml in 20 mM Tris, pH 8.0, on a rotator in coldroom
Uracil (1 ), ≥99% pure Bromoacetic acid, 97% pure Methanol (MeOH), ACS grade
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