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文献和实验Intracytoplasmic Spermatocyte Injection (ICSI)
[2]. Intracytoplasmatic sperm injection (ICSI) has raised hopes that these couples can have children of their own. In the meantime this method of treating male subfertility has achieved a breakthrough [3] and is gaining in importance. It is establishing
BAC DNA Purification For Injection
Equilibrate tip-500 column with 10 ml QBT buffer (750 mM NaCl, 50mM MOPS pH 7.0, 15% isopropanol, 0.15% Triton X-100) Apply filtered DNA solution to the column Wash column twice with 30 ml QC buffer (1.0 M NaCl, 50 mM MOPS pH 7.0, 15% isopropanol
ES injection pipette manufacture（图）
The following is an outline of the method that I use to manufacture injection needles for ES cell injection. I use high quality borosilicate glass obtained from World Precision Instruments (see vendor listing). This glass has an external
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