- ¥880
- abgent
- AP7237c
- 2026年02月23日
- IHC-P, WB, E
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 克隆性：
多克隆
- 适应物种：
Human, Mouse, Rat
- 供应商：
研卉生物
- 应用范围：
IHC-P, WB, E
- 抗体英文名：
SPHK1 Antibody (Center)
- 抗体名：
SPHK1 Antibody (Center)
- 规格：
80ul
Application IHC-P, WB, E
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Gene ID 8877
Other Names Sphingosine kinase 1, SK 1, SPK 1, SPHK1, SPHK, SPK
Target/Specificity This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human SPHK1.
Dilution IHC-P~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Name
| SPHK1 | |
| Synonyms | SPHK, SPK |
| Function | Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. |
| Cellular Location | Cytoplasm. Nucleus. Cell membrane. Note=Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner |
| Tissue Location | Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle |
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文献和实验Double immunohistochemistry for BCL6 and PNA for Germinal Center detection.
(see Reagents and Solutions). 3- incubate with the blocking for 10 min. If one of your antibody is biotin-conjugated, you need at this point to do endogenous biotin blocking. 4- blot the slides without washing and apply the primary antibody, in a moist
Double immunohistochemistry for BCL6 and PNA for Germinal Center detection
Double immunohistochemistry for BCL6 and PNA for Germinal Center detection. (on fixed, paraffin-embedded sections) Cut sections at 4µm, use a clean water bath with distilled water, and let the sections dry
with those with a postgerminal center/activated profile. Microarray technology may not be available in many diagnostic laboratories, and antibody-based methods are much simpler and cheaper and are therefore more applicable to the routine setting. Immunocytochemistry
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