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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
超亮全染色缓冲液
- 库存:
大量
- 保质期:
12个月
- 抗原来源:
其它
- 供应商:
上海研卉生物科技有限公司
- 是否单克隆:
多克隆
- 抗体英文名:
Super Bright Complete Staining Buffer, eBioscience
- 规格:
100μl
Super Bright Complete Staining Buffer, eBioscience
描述
Super Bright Complete Staining Buffer, eBioscience, is designed for use as a supplement to Flow Cytometry Staining Buffer in immunofluorescent staining protocols of cells in suspension. Super Bright Complete Staining Buffer is only necessary when using more than one polymer dye-conjugated antibody in the same sample to prevent non-specific polymer interactions, which can result in data appearing under-compensated. Super Bright Complete Staining Buffer is provided in a convenient 5 µL/test format and is compatible with traditional fluorochromes, Brilliant Violet dyes, and standard flow cytometry protocols.Super Bright Complete Staining Buffer is not compatible with UltraComp eBeads microspheres. If using UltraComp eBeads microspheres as a compensation tool, Flow Cytometry Staining Buffer should be used exclusively for all dilutions and washes during staining of the microspheres.
Super Bright Complete Staining Buffer is a fully compatible replacement for Super Bright Staining Buffer (Cat. Nos. SB-4400-42, SB-4400-75), and it shows decreased background in the 450/40 channel on the violet laser.
Super Bright polymer dyes are sold under license from Becton, Dickinson and Company.
规格
| Product Line: | eBioscience™ |
|---|---|
| Product Size: | 100 tests |
| Shipping Condition: | Wet Ice |
内容及储存
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文献和实验Intracellular Cytokine Staining Protocol
such as BSA or FCS can be added to the staining buffer. Optimal concentration of the fluorochrome-conjugated anti-cytokine antibodies has to be determined experimentally. To confirm specificity of the staining, it is common to block the directly-conjugated
Intracellular Immunofluorescent Staining for Flow Cytometry
ligand. Buffers used for intracellular staining can have varying effects. eBioscience antibodies are optimized using eBioscience Foxp3 staining buffer set (cat. 00-5523 ) or eBioscience IC Fixation Buffer (cat. 00-8222 ). All antibodies to cytokines
Antibody Staining of Dissected Gonads
to a small (6 mm (O.D.) x 35mm) glass culture tube (Kimax brand). Spin 1 min @ setting 2 in clinical benchtop centrifuge and remove super. Wash once with PBS. 2. Dilute primary and secondary antibodies in Blocking buffer and incubate as follows:
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