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文献和实验96-well RNA In Situ Hybridization Protocol
to the bottom of the well.IV. BUFFER COMPOSITION AND VENDOR INFORMATION 2X Polymerase mix 287μlddH2 0 100μl10X Boehringer M. transcription buffer 50μl10X dig-NTP mix 13μlRNase inhibitor (500U) 50μlT3 or T7 RNA polymerase (1000U) 500μlTotal Volume 10X dig NTP mix
96-well RNA In Situ Hybridization Protocol
embryos in a 50ml falcon tube). 2. Dechorionate 5 min. in 50% bleach. 3. Wash 3X with 0.02% Tx. 4. Fill tube with equal parts heptane and 4% paraformaldehyde/PBS (filtered). 5. Incubate 20 min. with frequent shaking. 6. Remove
1 µl of air. 6. Load 2 µl of Loading Buffer from the reservoir. 7. Place tips in clean wells of disposable mixing tray and allow pipette to mix the sample and loading dye. 8. Place the pipette tip in a 50 well row so that the tip
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