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低温
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鹿森生物
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特点
所需优化次数少
PCR特异性高
操作方便,可在室温下构建反应体系
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不同于抗体介导的方法,HotStarTaq DNA Polymerase应用化学介导的热启动,可确保聚合酶在PCR初始加热阶段完全没有活性。HotStarTaq DNA Polymerase提供独特的QIAGEN PCR Buffer,将非特异性扩增产物、引物二聚体和其他污染降至最低。一种新型的添加剂Q-Solution,可实现难扩增的模板(如GC含量高的模板)高效扩增。
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文献和实验Polymerase III in vitro Transcription
Polymerase III in vitro Transcription Steve Hahn For the following reactions, use appropriate shielding and dispose of radioactive waste properly! A 20 microliter transcription reaction contains: 4.0 μl 5X Pol III transcription buffer 0.2 μl
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
Dideoxy Sequencing Reactions Using Taq Polymerase
where ambiguities are frequently found on the gels (see Fig.1 ). Currently several companies have developed sequencing kits using Tuq DNA polymerase. We have tried the TAQenceTM (U.S. Biochemicals, Cleveland, OH) and the Tuq multiwellTM (Amersham, Arlington Heights
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