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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
DNA Methyltransferase 3a （Clone 64B1446） Antibody （DNMT3， DNMT3A）
- 克隆性：
多克隆
- 保存条件：
见包装
- 规格：
50 ug
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文献和实验of endogenous DNA.By the same token,a higher cellular protein concentration will also lead to a higher negative control for similar reasons ( protein methylation). If your positive controls are too low: Did you deviate from protocol? (e.g.,not drying
, we describe three DNA methyltransferase assays, each of them based on a different method: (1) An assay using radioactively labeled AdoMet and biotinylated DNA substrates that is ideal for enzymatic characterization of these enzymes. (2) An assay using bisulfite
in Econofluor) Also, a longer NaOH incubation might reduce this problem some. But remember, the higher the activity, the higher the negative control will be because of methylation of endogenous DNA. By the same token, a higher cellular protein concentration
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