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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见产品包装
- 保质期:
一年
- 英文名:
STAT1 (His-tagged), human recombinant
- 库存:
大量
- 供应商:
QIYBO
- 规格:
25ug
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文献和实验Signature-tagged mutagenesis (STM) was originally developed by David Holden while studying the filamentous fungus Aspergillus fumigatus . In attempts to define virulence determinants for this pathogenic fungus, candidate factors were selected
HIS-tagged protein purification
John Mundy,Institute of Molecular Biology,Copenhagen,Denmark 1 liter cell prep 1)grow 20ml cells O/N37℃ dilute 50X into prewarmed LB,grow to 0.6 OD or about 1hr.Induce w/ 2mM IPTG (238mg/0.5l),grow 3hr,spin 6k GS3 10',freeze at 70℃ 2)resuspend
Affinity Immobilization of Tagged Enzymes
for enzyme immobilization. This work describes experimental strategies for affinity immobilization of tagged enzymes onto activated supports. Protocols for attaching histidine (His) and sugar-tagged enzymes onto common supports such as commercial aminated
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