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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD3
- 亚型:
Mouse IgG2a
- 形态:
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
- 保存条件:
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
- 克隆性:
Monoclonal Antibody
- 标记物:
BIOT
- 适应物种:
Hu
- 宿主:
Mouse
- 应用范围:
FC
- 浓度:
Concentration:0.5 mg/ml
- 靶点:
Clone number:OKT-3
- 抗体英文名:
CD3 Antibody-BIOT;流式抗体
- 抗体名:
CD3 Antibody-BIOT;流式抗体
- 规格:
25/100 μg
克隆类型 :Mouse Monoclonal Antibody;Clone number:OKT-3
货 号 :HT1610633
规 格 :25/100 μg ;
亚 型 :
Mouse IgG2a
浓 度 :
Concentration:0.5 mg/ml
缓 冲 液 :
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
产品描述 :
CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. The OKT3 antibody is able to induce T cell activation.
适用物种 :
Hu
应用范围 :
FC
应用说明 :
The amount of the reagent is suggested to be used ≤ 1.0 μg / 100 µL of whole blood
保存条件 :
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
标 记 物 :
BIOT(其他荧光标记请咨询我们)
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文献和实验1 ml 2.4 中准备好的 Biotin Elution Buffer,让液流完全进入胶体后孵育 2 min,加入 9 ml 的 Biotin Elution Buffer; 1.13 对于从全血中分选目的细胞,再加入 10 ml 的 Biotin Elution Buffer; 1.14 300 g 转速离心 10 分钟,收集洗脱目的细胞。 流程图: 2. 实验数据 以下流式图为从 7.5 ml 全血中,选出 CD3 + T 细胞的数据。选后细胞以 CD3 -APC (OKT
CD3 分子 CD3 常用单克隆抗体或代号:T3,I.eu4,HCHTl 主要表达细胞:T,Thy T细胞 分子质量(kDa)和结构:γ,δ,ε,ζ,η分别为26,20, 19, 16, 2l 功 能:TCR/CD3复合体.T细胞信号转导 CD3 Aka OKT3 Function: complex (5 chains) of integral membrane
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
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