- ￥1700 - 4000
- GeneTex
- 美国
- GTX633631
- 2025年07月07日
- WB, ICC/IF, IHC-P
- Mouse
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human eIF3H / EIF3S3. The exact sequence is proprietary.
- 亚型：
IgG2b
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX633631
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
eIF3H / EIF3S3
- 抗体英文名：
eIF3H / EIF3S3 antibody [GT35512]
- 抗体名：
eIF3H / EIF3S3 抗体 [GT35512]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
eIF3H / EIF3S3 antibody [GT35512] detects eIF3H / EIF3S3 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
eIF3H / EIF3S3 antibody [GT35512] (GTX633631) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with eIF3H / EIF3S3 antibody [GT35512] (GTX633631) diluted at 1:1000.
eIF3H / EIF3S3 antibody [GT35512] detects eIF3H / EIF3S3 protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% PFA at RT for 15 min.
Green: eIF3H / EIF3S3 protein stained by eIF3H / EIF3S3 antibody [GT35512] (GTX633631) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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文献和实验Southern Blotting(Fred Hutchinson Cancer Research Cente
with Blot Wash #1: 2 x 5' at room temp (RT). 3. Wash with Blot Wash #2: 2 x 15' at 55℃. 4. Wash with Buffer 1: briefly (1') at RT. 5. (Optional; see above) Block with Buffer 2: 30' (O/N okay) Save and store at 4℃. 6. Incubate with antibody-conjugate
Phage‐Based Expression Cloning to Identify Interacting Proteins
the interacting protein. This technique leads directly to the isolation of a cDNA encoding the interacting protein, bypassing the need for labor?intensive protein purification, microsequencing, or antibody production.
: 500 mM Tris-HCl, pH 8.0, 150 mM MgCl2 in sterile double distilled water. 5 ml 1 M Tris-HCl, pH 8.0 1.5 ml 1 M MgCl2 sddH2O to 10 ml 50:2:10 TTE: 50 mM Tris-HCl, pH 8.0, 2% Triton X-100, and 10 mM EDTA in double distilled water
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