Pulsatilla saponin D (SB365) 可

Pulsatilla saponin D  (Synonyms: 白头翁皂苷D; SB365; Hederacolchiside A)

Pulsatilla saponin D (SB365) 可从 Pulsatilla chinensis根部分离得到，具有抗癌活性。

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生物活性    
Pulsatilla saponin D (SB365), isolated from the root of Pulsatilla chinensis, is an anti-tumor agent[1][2][3][4].

体外研究
(In Vitro)    
Pulsatilla saponin D (SB365) shows apoptotic effect accompanied by increased evidence of cleaved caspase-3 and poly (ADP ribose) polymerase[1].
Pulsatilla saponin D (SB365) strongly inhibits c-Met expression in gastric cancer cells[1].
Pulsatilla saponin D (SB365) strongly suppresses the growth and proliferation of 5 human pancreatic cancer cell lines (MIAPaCa-2, BXPC-3, PANC-1, AsPC-1 and HPAC) [2].
Pulsatilla saponin D (SB365) shows anti-angiogenic activity by decreasing the expression of HIF-1α and VEGF[3].
Pulsatilla saponin D (0.3 ng/mL to 10 μg/mL) exhibits anti-tumor activity in NSCLC cancer cells[4].

Cell Cytotoxicity Assay[4]

Cell Line:    A-549, SKMEL-2, MCF-7, and Lewis lung carcinoma (LLC) cells.
Concentration:    0.3 ng/mL to 10 μg/mL.
Incubation Time:    72 h.
Result:    Exhibited an ED50 value of 6.3 μg/mL for A-549 cells.

体内研究
(In Vivo)    
Pulsatilla saponin D (6.4 mg/kg) exhibits anti-tumor activity in Lewis lung carcinoma model[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:    2x2x2 mm3 tumor fragments of a Lewis lung carcinoma (LLC) transplanted s.c. into the auxiliary region of the BDF1 mice[4].
Dosage:    6.4 mg/kg.
Administration:    Intraperitoneally on the 1 st to 7 th and 9 th to 14 th day.
Result:    Had such a potent antitumor effect (IR, 82 %) on a solid tumor, which is higher than Adriamycin (IR, 64%).

分子量    
913.10

性状    
Solid

Formula    
C47H76O17

CAS 号    
68027-15-6

中文名称    
白头翁皂苷 D

结构分类    
TerpenoidsTriterpenes
来源    
PlantsRanunculaceaePulsatilla chinensis (Bunge) Regel
运输条件    
Room temperature in continental US; may vary elsewhere.

储存方式    
4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据    
In Vitro: 
DMSO : ≥ 39 mg/mL (42.71 mM)

* "≥" means soluble, but saturation unknown.

配制储备液    
浓度溶剂体积质量    1 mg    5 mg    10 mg
1 mM    1.0952 mL    5.4759 mL    10.9517 mL
5 mM    0.2190 mL    1.0952 mL    2.1903 mL
10 mM    0.1095 mL    0.5476 mL    1.0952 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

Solubility: ≥ 2.08 mg/mL (2.28 mM); Clear solution

2.
请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.08 mg/mL (2.28 mM); Clear solution

3.
请依序添加每种溶剂： 10% DMSO    90% corn oil

Solubility: ≥ 2.08 mg/mL (2.28 mM); Clear solution
 

参考文献

[1]. Hong SW, et al. SB365, Pulsatilla saponin D, targets c-Met and exerts antiangiogenic and antitumor activities. Carcinogenesis. 2013 Sep;34(9):2156-69.  [Content Brief] [2]. Son MK, et al. SB365, Pulsatilla saponin D suppresses proliferation and induces apoptosis of pancreatic cancer cells. Oncol Rep. 2013 Aug;30(2):801-8.  [Content Brief] [3]. Son MK, et al. SB365, Pulsatilla saponin D suppresses the proliferation of human colon cancer cells and induces apoptosis by modulating the AKT/mTOR signalling pathway. Food Chem. 2013 Jan 1;136(1):26-33.  [Content Brief] [4]. Yong Kim, et al. Pulsatilla saponin D: the antitumor principle from Pulsatilla koreana. Arch Pharm Res. 2004 Sep;27(9):915-8.  [Content Brief]

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