Clomipramine hydrochloride 有效阻

Clomipramine (Chlorimipramine) hydrochloride is a potent 5-HT reuptake blocker with the IC₅₀ value of 1.5 nM. Clomipramine hydrochloride is a tricyclic antidepressant that can be used for the research of depression and obsessive compulsive disorder (OCD)^[1].

Clomipramine hydrochloride can inhibit reuptake of both noradrenaline and 5-HT, although Clomipramine inhibits 5-HT reuptake more strongly than it inhibits noradrenaline reuptake^[1].
The antidepressant Clomipramine inhibits both venom AChE as well as human serum BChE in a concentration-dependent manner but has no effect on AChE in the rat brain striatum^[2]. Clomipramine hydrochloride interferes with the autophagic flux and severely compromises the viability of tumorigenic cells upon cytotoxic stress^[3].
Clomipramine hydrochloride reduces autophagy in neuronal primary cultures. Clomipramine (1 and 5 µM) hydrochloride negatively regulates neuronal autophagic pathway in primary cultured cells^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Clomipramine (5-20 mg/kg; i.p) hydrochloride elicits significant hyperglycemia in mice. Clomipramine hydrochloride induces hyperglycemia in mice by blocking the 5-HT2B and/or 5-HT2C receptors, which results in facilitation of adrenaline release. In mice, Clomipramine hydrochloride reduces immobility in the forced swimming test, which is the behavioral model for antidepressants. Clomipramine hydrochloride also inhibits the OCD animal model, marble burying behavior in mice^[1]. Clomipramine (20 mg/kg) hydrochloride decreases autophagic flux in murine tissues^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

351.31

C₁₉H₂₄Cl₂N₂

17321-77-6

盐酸氯米帕明；盐酸氯丙咪嗪

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

H₂O : ≥ 50 mg/mL (142.32 mM)

DMSO : 50 mg/mL (142.32 mM; Need ultrasonic)

* "≥" means soluble, but saturation unknown.

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： PBS

  Solubility: 100 mg/mL (284.65 mM); Clear solution; Need ultrasonic

• 2.

  请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline

  Solubility: ≥ 3 mg/mL (8.54 mM); Clear solution

• 3.

  请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)

  Solubility: ≥ 3 mg/mL (8.54 mM); Clear solution

• 4.

  请依序添加每种溶剂： 10% DMSO 90% corn oil

  Solubility: ≥ 3 mg/mL (8.54 mM); Clear solution

• [1]. Yumi Sugimoto, et al. The tricyclic antidepressant Clomipramine increases plasma glucose levels of mice. J Pharmacol Sci. 2003 Sep;93(1):74-9. [Content Brief]

  [2]. Mushtaq Ahmed, et al. Comparative study of the inhibitory effect of antidepressants on cholinesterase activity in Bungarus sindanus (krait) venom, human serum and rat striatum. J Enzyme Inhib Med Chem. 2008 Dec;23(6):912-7. [Content Brief]

  [3]. Federica Cavaliere,The tricyclic antidepressant Clomipramine inhibits neuronal autophagic flux. Sci Rep. 2019 Mar 19;9(1):4881. [Content Brief]