- ￥900 - 1800
- 华拓生物
- 中国
- HT1610507
- 2025年07月15日
- FC
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
IFN-γ
- 亚型：
Rat IgG1
- 形态：
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
- 保存条件：
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
- 克隆性：
Monoclonal Antibody
- 标记物：
PE-CY5
- 适应物种：
Mouse
- 宿主：
Rat
- 应用范围：
FC
- 浓度：
Concentration：0.2 mg/ml
- 靶点：
Clone number：XMG1.2
- 抗体英文名：
IFN-γ Antibody-PE-CY5；流式抗体
- 抗体名：
IFN-γ Antibody-PE-CY5；流式抗体
- 规格：
25/100 μg
克隆类型 ：Rat Monoclonal Antibody；Clone number：XMG1.2
货 号 ：HT1610507
规 格 ：25/100 μg ;
亚 型 ：
Rat IgG1
浓 度 ：
Concentration：0.2 mg/ml
缓 冲 液 ：
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
产品描述 ：
Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory,and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The XMG1.2 antibody reacts with mouse interferon-γ (IFN-γ). The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.
适用物种 ：
Mouse
应用范围 ：
FC
应用说明 ：
The amount of the reagent is suggested to be used ≤ 1.0 µg /10^6 cells in 100 µl
保存条件 ：
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
标 记 物 ：
PE-CY5（其他荧光标记请咨询我们）
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文献和实验Protocol for intracytoplasmic staining of cytokines for FACS analysis
analysis: anti-IL2 (S4B6-FITC; Pharmingen 18004A or home made conjugate) for FL1 anti-TNF-alpha (MP6-XT22-PE; Pharmingen 18135A) for FL2 anti-IFN-gamma (XMG1.2-FITC; Pharmingen 18114A or home made conjugate) for FL1 anti-IL4 (11B11-PE
Dual- and Triple-Color Fluorospot
3, and Cy5. 3. Fluorescence Enhancer (Mabtech) is used to improve the fluorescent signal. 实验步骤 The protocol describes double staining for the detection of human IFN-γ and IL-2. PVDF plates are coated with mAbs to IFN-γ and IL
Intracellular Immunofluorescent Staining for Flow Cytometry
mouse spleen ConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d) 2d/3d anti-CD3 (10ug/ml immobilized) + anti-CD28 (2ug/ml soluble) 5hr Brefeldin A XMG1.2 IL-1α mouse
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