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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD278 (ICOS)
- 亚型:
Rat IgG2b
- 形态:
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
- 保存条件:
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
- 克隆性:
Monoclonal Antibody
- 标记物:
FITC
- 适应物种:
Mouse
- 宿主:
Rat
- 应用范围:
FC
- 浓度:
Concentration:0.5 mg/ml
- 靶点:
Clone number:17G9
- 抗体英文名:
CD278 (ICOS) Antibody-FITC;流式抗体
- 抗体名:
CD278 (ICOS) Antibody-FITC;流式抗体
- 规格:
50/500 μg
克隆类型 :Rat Monoclonal Antibody;Clone number:17G9
货 号 :HT1610405
规 格 :50/500 μg ;
亚 型 :
Rat IgG2b
浓 度 :
Concentration:0.5 mg/ml
缓 冲 液 :
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
产品描述 :
The 17G9 antibody reacts with the 47-57 kD ICOS protein, also known as inducible costimulatory molecule, and H4. This protein is homologous to the CD28/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes and can costimulate T cells and induce proliferation. In addition ICOS has been shown to be involved in humoral immune responses (B cell germinal center formation). The ICOS ligand, B7h/B7RP-1 and B7-H2 is constitutively expressed in B cell areas of secondary lymphoid organs and can be induced in other tissues by LPS. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development. ICOS stimulation has been shown to be involved in airway tolerance and the downregulation of pulmonary inflammation.
适用物种 :
Mouse
应用范围 :
FC
应用说明 :
The amount of the reagent is suggested to be used ≤ 0.5 µg /10^6 cells in 100 µl
保存条件 :
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
标 记 物 :
FITC(其他荧光标记请咨询我们)
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文献和实验CD3/ CD4 /CD8 T cell subset counts
Description Procedure for CD3/ CD4 /CD8 T cell subset counts using CD3-FITC / CD4-PE / CD8-PECy5 antibody Procedure 1. One 5 ml round bottom tube (Falcon) is taken per blood sample to be analyzed. The tube is labeled, which includes
的MYPPPY基序是与B7分子结合的部位。CD28分子的胞质区带有ITAM,其结构虽然和CD3℃链上的ITAM略有不同,但参与传递T细胞活化的第二信号。 近年来研究发现,T细胞除表达CD28和CTLA4外,还有多种CD28家族新的成员。 ①诱导性协同刺激分子(1COS),即CD278,在氨基酸水平上与CD28分子有39%同源性,主要表达于活化Th2细胞。ICOS的配体ICOS-L即B7―H2(CD275)。ICOS主要调节Th2细胞的功能,诱导IL-10产生,促进B细胞分化、记忆B
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
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