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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
CD3
- 亚型:
Rat IgG2b
- 形态:
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
- 保存条件:
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
- 克隆性:
Monoclonal Antibody
- 标记物:
BIOT
- 适应物种:
Mouse
- 宿主:
Rat
- 应用范围:
FC
- 浓度:
Concentration:0.5 mg/ml
- 靶点:
Clone number:17A2
- 抗体英文名:
CD3 Antibody-BIOT;流式抗体
- 抗体名:
CD3 Antibody-BIOT;流式抗体
- 规格:
50/500 μg
克隆类型 :Rat Monoclonal Antibody;Clone number:17A2
货 号 :HT1610017
规 格 :50/500 μg ;
亚 型 :
Rat IgG2b
浓 度 :
Concentration:0.5 mg/ml
缓 冲 液 :
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
产品描述 :
CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
适用物种 :
Mouse
应用范围 :
FC
应用说明 :
The amount of the reagent is suggested to be used ≤ 0.25 µg /10^6 cells in 100 µl
保存条件 :
Store at 4°C and protected from prolonged exposure to light. Do not freeze.
标 记 物 :
BIOT(其他荧光标记请咨询我们)
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文献和实验T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
transfer of residual antibody. Vials of Dynabeads® CD3⁄CD28 CTS™ are at negative pressure – liquid must be displaced with air and vice versa. 2) Place one 10 ml vial of Dynabeads® CD3⁄CD28 CTS™ on the MPC-1 for 1 min. 3)
Th17 Polarization of Mouse CD4 Cells
实验试剂 Sterile PBS Cell culture medium Sterile plastic petridishes RBC Lysis Buffer [Details: Biolengend, cat.#420301] Anti-mouse CD3ε, clone145-2C11[Details: LEAF™format, cat.#100314]
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