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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详见说明
- 保质期:
详见说明
- 英文名:
2 x SYBR Green qPCR Mix(With 100 x ROX)
- 库存:
充足
- 供应商:
杭州昊鑫生物
- 规格:
125 次 x 20μl反应体系
艾德莱浙江省总代理-杭州昊鑫生物
北京艾德莱生物科技有限公司致力于为生命科学研究人员提供简单、易用、值得信赖的分子生物学和生物化学试剂类产品以及专业的技术服务。同时公司针对生物试剂行业规模小,标准低,批次之间产品稳定性差的弊端,引入生物医药公司风险投资作为战略合作伙伴,将生物医药级的规模生产设备、工艺、流程等引入科研试剂领域,提供具有医药级质量标准的科研试剂。公司未来将立足生物技术前沿领域,不断推出质量稳定、价格合理的新产品,与广大科研工作者携手为中国生命科学发展不断努力。

|
货号#
|
规格
|
价格
|
|
PC5901
|
125 次 x 20μl反应体系
|
400.00 元
|
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PC5902
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500 次 x 20μl反应体系
|
1300.00 元
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储存:-20 ℃ 避光保存至少12个月,使用前充分融解混匀。短期使用可放在4 ℃,避免反复冻融。
制品说明:
注意事项:
1. 本制品含独立包装参比染料ROX(货号为PC38 Rox Reference Dye),客户并根据qPCR仪器技术指导决定是否需要加ROX参比染料和加入浓度,用于消除信号本底以及校正孔与孔之间产生的荧光信号误差。
使用该产品发表的部分文章:http://www.aidlab.cn/news-show.asp?id=105
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文献和实验1. Qingkailing Injection Alleviates Experimental Autoimmune Uveitis in Rats via Inhibiting Th1 and Th17 Effector Cells. Biol. Pharm. Bull, 2012, 35(11) : 1991–1996
2. Expression Analysis of MAwuAG in Different Organs and Developmental Stages of Magnolia wufengensis. Chinese Bulletin of Botany, 2013, 48 (2): 1–1
3. Effect and Molecular Mechanism of DHA on Improving Rats Learning and Memory Ability, Food Science, 2012-11-9
4. Zinc Oxide Nanoparticles Induce Rat Retinal Ganglion Cell Damage Through Bcl-2,Caspase-9 and Caspase-12 Pathways Journal of Nanoscience and Nanotechnology. Vol. 13, 3769–3777, 2013
5. Zinc oxide nanoparticles decrease the expression and activity ofplasma membrane calcium ATPase, disrupt the intracellular calciumhomeostasis in rat retinal ganglion cells. The International Journal of Biochemistry & Cell Biology 45 (2013) 1849–1859
6. Zinc oxide nanoparticles inhibit Ca2+-ATPase expression in human lens epithelial cells under UVB irradiation. Toxicology in Vitro 27 (2013) 2117–2126
7. The organic extract from the Securidaca inhibits duck hepatitis B virus replication in vivo and hepatoprotective effect. Chin J Clinicians(Electronic Edition),December 1,2013,Vol.7,No.23
8. Disrupted calcium homeostasis is involved in elevated zinc ion-induced photoreceptor cell death. Archives of Biochemistry and Biophysics 2014, 560: 44–51
9. Molecular cloning and expression analysis of a myosin light chain 1 (MLC-1) gene from Indian meal moth Plodia interpunctella (Lepidoptera: Pyralidae). Entomological Research 2015, 45: 305-313
10.miR-143 inhibits tumor progression by targeting FAM83F in esophageal squamous cell carcinomaTumor Biol. 2016, DOI 10.1007/s13277-015-4760-9
11.An adenine nucleotide translocase (ANT) gene from Apostichopus japonicus; molecular cloning and expression analysis in response to lipopolysaccharide (LPS) challenge and thermal stress. Fish & Shellfish Immunology 2016, 49: 16 - 23
12.The Y137H mutation of VvCYP51 gene confers the reduced sensitivity to tebuconazole in Villosiclava virens. Scientific Reports 2015, 5:17575 | DOI: 10.1038/srep17575
13.Effects of the ninein like protein centrosomal protein on breast cancer cell invasion and migration. MOLECULAR MEDICINE REPORTS 2015, 12: 1659-1664
14.Molecular characterization of a 14-3-3 zeta gene from Plodia interpunctella: A potential marker for phylogenetic inference. Biochemical Systematics and Ecology 2015, 60: 171-176
15.MicroRNA-373 promotes migration and invasion in human esophageal squamous cell carcinoma by inhibiting TIMP3 expression. Am J Cancer Res 2016, 6(1):1-14
16.Development and Application of a Quantitative RT-PCR Approach for Quantification of Grapevine fanleaf virus. Acta Horticulturae Sinica 2016, 43 (3): 538-548.
17.Improved ganoderic acids production in Ganoderma lucidum by wood decaying components. Scientific Reports 2017, 7:46623 | DOI: 10.1038/srep46623
18.Identification of differentially expressed genes in the spleens of polyriboinosinic polyribocytidylic acid (poly I:C)-stimulated yellow catfish Pelteobagrus fulvidraco. Fish & Shellfish Immunology 2016, 56: 278-285.
19.Molecular cloning and expression analysis of the highly conserved eukaryotic translation initiation factor 5A (eIF‐5A) from Antheraea pernyi. Entomological research 2018, 48(1): 11-17.
20.A small heat shock protein 21 (sHSP21) mediates immune responses in Chinese oak silkworm Antheraea pernyi. International Journal of Biological Macromolecules 2018, 111: 1027-1031.
21.The Research on the Relationship of RAGE, LRP-1, and Aβ Accumulation in the Hippocampus, Prefrontal Lobe, and Amygdala of STZ-Induced Diabetic Rats. Journal of Molecular Neuroscience 2017, 62(1): 1–10.
22.Relationships of BRAF mutation and HMGB1 to papillary thyroid carcinoma. Biochemical and Biophysical Research Communications 2017, 486(4): 898-903.
23.nTranscriptome analysis of yellow catfish (Pelteobagrus fulvidraco) liver challenged with polyriboinosinic polyribocytidylic acid (poly I:C). Fish & Shellfish Immunology 2017, 68: 395-403.
24.Overexpression of lncRNA DANCR positively affects progression of glioma svia activating Wnt/β-catenin signaling. Biomedicine & Pharmacotherapy 2018, 102: 602-607.
25.Biochemical and genetic toxicity of dinotefuran on earthworms (Eisenia fetida). Chemosphere 2017, 176: 156-164.
26.Oxidative stress and gene expression of earthworm (Eisenia fetida) to clothianidin Ecotoxicology and Environmental Safety 2017, 142: 489-496.
27.De novo transcriptome assembly and analysis of differential gene expression following lipopolysaccharide challenge in Pelteobagrus fulvidraco. Fish & Shellfish Immunology 2018, 73: 84-91.
28.Autophagy-lysosome dysfunction is involved in Aβ deposition in STZ-induced diabetic rats. Behavioural Brain Research 2017, 320: 484-493.
29.Molecular identification and expression analysis of a goose-type lysozyme (LysG) gene in yellow catfish Pelteobagrus fulvidraco. Fish & Shellfish Immunology 2016, 58: 423-428.
30.A study of the damage of the intestinal mucosa barrier structure and function of Ctenopharyngodon idella with Aeromonas hydrophila. Fish Physiology and Biochemistry 2017, 43(5): 1223–1235.
31.Immunomodulatory effects of Longdan Xiegan Tang on CD4+/CD8+ T cells and associated inflammatory cytokines in rats with experimental autoimmune uveitis. Molecular Medicine Reports 2016, : 2746-2754.
32.De novo transcriptome assembly and analysis of differential gene expression following peptidoglycan (PGN) challenge in Antheraea pernyi. International Journal of Biological Macromolecules 2018, 112: 1199-1207.
33.Zinc ions regulate opening of tight junction favouring efflux of macromolecules via the GSK3β/snail-mediated pathway. Metallomics 2018, 10: 169-179.
34.Myeloid leukemia factor functions in anti-WSSV immune reaction of kuruma shrimp, Marsupenaeus japonicus. Fish & Shellfish Immunology 2017, 70: 416-425.
35.Gene microarray analysis of lncRNA and mRNA expression profiles in patients with high grade ovarian serous cancer. International Journal of Molecular Medicine 2018: 91-104.
36.Assessing the influence of 1-dodecyl-3-methylimidazolium chloride on soil characteristics and Vicia faba seedlings. Ecotoxicology and Environmental Safety 2018, 152: 114-120.
37.Linc-ROR induces epithelial-to-mesenchymal transition in ovarian cancer by increasing Wnt/β-catenin signaling. Oncotarget 2017, 8(41): 69983–69994.
38.Targeting the HMGA2 oncogene by miR-498 inhibits non-small cell lung cancer biological behaviors. European Review for Medical and Pharmacological Sciences 2018, 22: 1693-1699.
几个复孔,选择重复性好的 CT 值参与计算。 (2)CT<30,重复性较差。这种情况一般同操作有关。 解决办法:从以下几个方面进行问题的排查:①加样准确度;②移液器吸取液体的准确度;③定期校准 qPCR 仪。 ①加样准确度 避免小体积加样,减少加样误差。可以将引物、SYBR Green Mix、ddH2O 配置成混合体系,并且增加模板的稀释梯度,大体积加样。如:原液 cDNA 添加 1μl,可以更改为原液 cDNA 稀释 5 倍,添加 5μl,使用 ddH2O 补齐体系至 20μl 即可
PCR产物的荧光值就可以。参比染料的作用是标准化荧光定量反应中的非PCR震荡,校正加样误差或者是孔与孔之间的误差,提供一个稳定的基线。现在很多公司已经把ROXTM配制在MasterMix或者Premixture里。如果反应曲线良好或已经优化好反应体系,也可以不加ROXTM染料校正。通常来讲,real-time qPCR的反应程序不需要像常规的PCR那样,要变性、退火、延伸3步。由于其产物长度在80-150bp 之间,所以只需要变性和退火就可以了。SYBR@Green等染料法,最好在PCR扩增程序结束
SYBR Green Quantitative PCR Protocol
the relative values for your gene of interest’s change in expression. SYBR qPCR Quick Protocol StandardsØ Dilute the cDNA ~4-fold (e.g. 21μl sample + 59μl H2 O = 80μl)Ø Pool an appropriate amount from each sample to create standard 1 (i.e. 30μl x
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