手机验证
充足
杭州昊鑫生物
15.6-1,000pg/mL
双抗夹心
详见说明
Canis familiaris; Canine (Dog,犬)
详见说明
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
48T
本试剂盒用于检测肿瘤坏死因子α(TNFa),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
分别于定值血清及血浆样本中加入一定量的肿瘤坏死因子α(TNFa)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 78-97 | 85 |
EDTA plasma(n=5) | 78-95 | 81 |
heparin plasma(n=5) | 92-101 | 97 |
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
在定值血清及血浆样本内加入适量的肿瘤坏死因子α(TNFa),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中肿瘤坏死因子α(TNFa)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 81-91% | 78-101% | 92-99% | 78-99% |
EDTA plasma(n=5) | 78-95% | 88-102% | 87-95% | 85-97% |
heparin plasma(n=5) | 94-101% | 80-92% | 95-103% | 98-105% |
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。
将肿瘤坏死因子α(TNFa)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的肿瘤坏死因子α(TNFa)与连接于固相载体上的抗体结合,然后加入生物素化的肿瘤坏死因子α(TNFa)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的肿瘤坏死因子α(TNFa)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。
杂志 | 参考文献 |
Experimental Animals | Anti-Inflammatory Effect of Guan-Xin-Er-Hao via the Nuclear Factor-Kappa B Signaling Pathway in Rats with Acute Myocardial Infarction [Jstage: source] |
Not available | Analysis of genetic markers for cardiovascular disorders in a portuguese population with familial hypercholesterolemia [Universi: 49434630] |
41 | Deficiency of Rac1 Blocks NADPH Oxidase Activation, Inhibits Endoplasmic Reticulum Stress, and Reduces Myocardial Remodeling in a Mouse Model of Type 1 Diabetes. [PubMed: 20522592] |
Respiration | Protective Effect of Nicotine on Lipopolysaccharide-Induced Acute Lung Injury in Mice [Karger: 000319151] |
Journal of Huazhong University of Science and Technology-Medical Sciences | Expression and significance of fgl2 prothrombinase in cardiac microvascular endothelial cells of rats with type 2 diabetes [SpringerLink: 161m725t24x85kvv] |
The European Journal of Clinical Investigation | Mesangial medium with IgA1 from IgA nephropathy inhibits nephrin expression in mouse podocytes [Wiley: source] |
Journal of Biomedical Research | Fibrinogen-like protein 2 expression correlates with microthrombosis in rats with type 2 diabetic nephropathy [ScienceDirect: S1674830111600158] |
Stem Cells | Combined Effects of Hematopoietic Progenitor Cell Mobilization from Bone Marrow by Granulocyte Colony Stimulating Factor and AMD3100 and Chemotaxis into the Brain Using Stromal Cell-Derived Factor-1α in an Alzheimer's Disease Mouse Model [PubMed: 21608078] |
Journal of Physiology and Biochemistry | Protective effects of curcumin, α-lipoic acid, and N-acetylcysteine against carbon tetrachloride-induced liver fibrosis in rats [SpringerLink: h875w73171w2147w] |
The EMBO Journal | Egr-1 decreases adipocyte insulin sensitivity by tilting PI3K/Akt and MAPK signal balance in mice [Nature: 2011277] |
Reproductive BioMedicine Online | Soluble BAFF-R produced by decidual stromal cells plays an inhibitory role in monocytes and macrophages [ScienceDirect: S1472648312001484] |
Journal of Vascular and Interventional Radiology | Hepatocellular Necrosis, Apoptosis, and Proliferation after Transcatheter Arterial Embolization or Chemoembolization in a Standardized Rabbit Model [PubMed: 21959058] |
Bone | Synthesis and inflammatory response of a novel silk fibroin scaffold containing BMP7 adenovirus for bone regeneration [PubMed: 22796416] |
Journal of Periodontology | The Effects of a Novel Botanical Agent on LPS-Induced Alveolar Bone Loss in Rats [PubMed: 23106504] |
Ann Rheum Dis. | The cathelicidins LL-37 and rCRAMP are associated with pathogenic events of arthritis in humans and rats [PubMed: 23172753] |
Journal of Surgical Research | Enhanced tumor growth after portal vein embolization in a rabbit tumor model [PubMed: 23149224] |
Journal of Applied Physiology | Simvastatin attenuates sympathetic hyperinnervation to prevent atrial fibrillation during the postmyocardial infarction remodeling process [PubMed: 22984252] |
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杂志 | 参考文献 |
Experimental Animals | Anti-Inflammatory Effect of Guan-Xin-Er-Hao via the Nuclear Factor-Kappa B Signaling Pathway in Rats with Acute Myocardial Infarction [Jstage: source] |
Not available | Analysis of genetic markers for cardiovascular disorders in a portuguese population with familial hypercholesterolemia [Universi: 49434630] |
41 | Deficiency of Rac1 Blocks NADPH Oxidase Activation, Inhibits Endoplasmic Reticulum Stress, and Reduces Myocardial Remodeling in a Mouse Model of Type 1 Diabetes. [PubMed: 20522592] |
Respiration | Protective Effect of Nicotine on Lipopolysaccharide-Induced Acute Lung Injury in Mice [Karger: 000319151] |
Journal of Huazhong University of Science and Technology-Medical Sciences | Expression and significance of fgl2 prothrombinase in cardiac microvascular endothelial cells of rats with type 2 diabetes [SpringerLink: 161m725t24x85kvv] |
The European Journal of Clinical Investigation | Mesangial medium with IgA1 from IgA nephropathy inhibits nephrin expression in mouse podocytes [Wiley: source] |
Journal of Biomedical Research | Fibrinogen-like protein 2 expression correlates with microthrombosis in rats with type 2 diabetic nephropathy [ScienceDirect: S1674830111600158] |
Stem Cells | Combined Effects of Hematopoietic Progenitor Cell Mobilization from Bone Marrow by Granulocyte Colony Stimulating Factor and AMD3100 and Chemotaxis into the Brain Using Stromal Cell-Derived Factor-1α in an Alzheimer's Disease Mouse Model [PubMed: 21608078] |
Journal of Physiology and Biochemistry | Protective effects of curcumin, α-lipoic acid, and N-acetylcysteine against carbon tetrachloride-induced liver fibrosis in rats [SpringerLink: h875w73171w2147w] |
The EMBO Journal | Egr-1 decreases adipocyte insulin sensitivity by tilting PI3K/Akt and MAPK signal balance in mice [Nature: 2011277] |
Reproductive BioMedicine Online | Soluble BAFF-R produced by decidual stromal cells plays an inhibitory role in monocytes and macrophages [ScienceDirect: S1472648312001484] |
Journal of Vascular and Interventional Radiology | Hepatocellular Necrosis, Apoptosis, and Proliferation after Transcatheter Arterial Embolization or Chemoembolization in a Standardized Rabbit Model [PubMed: 21959058] |
Bone | Synthesis and inflammatory response of a novel silk fibroin scaffold containing BMP7 adenovirus for bone regeneration [PubMed: 22796416] |
Journal of Periodontology | The Effects of a Novel Botanical Agent on LPS-Induced Alveolar Bone Loss in Rats [PubMed: 23106504] |
Ann Rheum Dis. | The cathelicidins LL-37 and rCRAMP are associated with pathogenic events of arthritis in humans and rats [PubMed: 23172753] |
Journal of Surgical Research | Enhanced tumor growth after portal vein embolization in a rabbit tumor model [PubMed: 23149224] |
Journal of Applied Physiology | Simvastatin attenuates sympathetic hyperinnervation to prevent atrial fibrillation during the postmyocardial infarction remodeling process [PubMed: 22984252] |