低氧诱导因子1α(HIF1a)检测试剂盒(酶联免疫吸附试验法

低氧诱导因子1α(HIF1a)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a)

HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78

优尔生一级代理：杭州昊鑫生物
产品更多信息请咨询
优尔生位于武汉出口加工区，已整体通过ISO 9001:2008、ISO 13485:2003质量管理体系认证，以提供生命科学科研用检测试剂为主，75%以上的服务对象位于欧美国家。凭借着深厚的技术积累和庞大的引物、cDNA、质粒、杂交瘤细胞“种子库”和检测试剂半成品库库存，能够做到约11,000种蛋白、19,000种抗体以及7,000种检测试剂盒立等可取。蛋白项目部拥有四大技术平台，包括多肽合成平台、小分子抗原性改造平台、天然蛋白提取平台和采用原核（E.Coli）表达系统与真核（酵母、杆状病毒-昆虫细胞、哺乳动物细胞）表达系统的重组蛋白表达平台。抗体项目部拥有完备的单克隆抗体和多克隆抗体制备平台。免疫应用部负责检测试剂盒的研制，主要是ELISA和CLIA试剂盒。成品检测部负责所有蛋白、抗体以及检测试剂盒产成品的检测及质量控制。公司实行三级质量控制，包括原料检测、半成品检测及成品检测。
 

• 编号SEA798Po
• 物种Sus scrofa; Porcine (Pig，猪) 相同的名称，不同的物种。
• 实验方法双抗夹心
• 反应时长3h
• 检测范围15.6-1,000pg/mL
• 灵敏度最小可检测剂量小于等于6.4pg/mL.
• 样本类型serum, plasma, tissue homogenates and other biological fluids
• 下载英文说明书   中文说明书
• 规格48T96T 96T*5 96T*10 96T*100
• 价格¥ 3175

产品包装图模拟

特异性

本试剂盒用于检测低氧诱导因子1α(HIF1a)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的低氧诱导因子1α(HIF1a)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本	回收率范围(%)	平均回收率(%)
serum(n=5)	91-101	96
EDTA plasma(n=5)	84-99	90
heparin plasma(n=5)	88-101	94

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100 
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10% 
批间差： CV<12% 

线性

在定值血清及血浆样本内加入适量的低氧诱导因子1α(HIF1a)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中低氧诱导因子1α(HIF1a)含量的测定值与理论值的比率。

样本	1:2	1:4	1:8	1:16
serum(n=5)	87-97%	78-103%	89-97%	91-98%
EDTA plasma(n=5)	94-102%	79-101%	86-94%	88-98%
heparin plasma(n=5)	81-102%	82-97%	79-102%	78-102%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将低氧诱导因子1α(HIF1a)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的低氧诱导因子1α(HIF1a)与连接于固相载体上的抗体结合，然后加入生物素化的低氧诱导因子1α(HIF1a)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的低氧诱导因子1α(HIF1a)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

 

参考文献

杂志	参考文献
Cancer Chemotherapy and Pharmacology	Quercetin greatly improved therapeutic index of doxorubicin against 4T1 breast cancer by its opposing effects on HIF-1α in tumor and normal cells. [PubMed: 19466611]
European Journal of Pharmacology	Baicalin suppresses lung carcinoma and lung metastasis by SOD mimic and HIF-1α inhibition [ScienceDirect: S0014299909011352]
PLoS ONE	Lactate Dehydrogenase 5 Expression in Non-Hodgkin Lymphoma Is Associated with the Induced Hypoxia Regulated Protein and Poor Prognosis [PubMed: PMC3781153]
International Journal of Molecular Medicine	Selective inhibition of PKCβ2 preserves cardiac function after myocardial infarction and is associated with improved angiogenesis of ischemic myocardium in diabetic rats [Pubmed: 24002353]
Canadian Journal of Physiology and Pharmacology	Modulation of arginine and asymmetric dimethylarginine concentrations in liver and plasma by exogenous hydrogen sulfide in LPS-induced endotoxemia [Pubmed: 24289078]
US8569372 B2	Method for preventing or treating a disease, disorder or condition induced by retina ischemia [US8569372]
J Surg Res.	Effects of adrenomedullin and vascular endothelial growth factor on ischemia/reperfusion injury in skeletal muscle in rats. [Pubmed: 23816242]
PLoS One.	HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa Keratitis [Plos: Source]
Journal of Physiological Anthropology	Telomeres are elongated in rats exposed to moderate altitude [Pubmed:Pmc4088304]
Molecular Medicine Reports	Resveratrol abrogates the effects of hypoxia on cell proliferation, invasion and EMT in osteosarcoma cells through downregulation of the HIF-1α protein. [Pubmed:25384583]
International Journal of Pharmaceutics	The enhancement of gene silencing efficiency with chitosan-coated liposome formulations of siRNAs targeting HIF-1α and VEGF [Pubmed:25445537]
International Journal of Molecular Medicine	Effect of combined treatment with rosuvastatin and protein kinase Cβ2 inhibitor on angiogenesis following myocardial infarction in diabetic rats [Pubmed:25524396]
International Journal of Developmental Neuroscience	Maternal omega-3 fatty acid supplementation to a vitamin B 12 deficient diet normalizes angiogenic markers in the pup brain at birth [PubMed: 25889224]
Eur Surg Res	Determination of the Preferred Conditions for the Isolated Perfusion of Porcine Kidneys [PubMed: 25341590]
Sci Rep	Oxygen-induced cell migration and on-line monitoring biomarkers modulation of cervical cancers on a microfluidic system [PubMed: 25905434]
Evidence-Based Complementary and Alternative Medicine	Metabolomic Analysis of Biochemical Changes in the Plasma of High-Fat Diet and Streptozotocin-Induced Diabetic Rats after Treatment with Isoflavones Extract of Radix Puerariae [pmc:PMC4794592]
Biol Trace Elem Res.	Roles of Copper in Hepatocarcinogenesis via the Activation of Hypoxia-Inducible Factor-1α [Pubmed:27121973]
Раны и раневые инфекции.	Использование гипоксического прекондиционирования для подготовки аутодермотрансплантата у больных со скомпрометированной микроциркуляцией [91]
Biological Trace Element Research	Roles of Copper in Hepatocarcinogenesis via the Activation of Hypoxia-Inducible Factor-1α. [pubmed:27121973]
Experimental and Therapeutic Medicine	Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis [etm:13]
Clinical Trials in Degenerative Diseases	Correlating oxidative stress-related factors with bone metabolic markers in older adult male patients exhibiting degenerative osteoporosis in the high-altitude hypoxic area of China: study protocol for a non-randomized controlled trial [issn:2542-3975]
Annals of Clinical & Laboratory Science	R132H Mutation in IDH1 Gene is Associated with Increased Tumor HIF1-Alpha and Serum VEGF Levels in Primary Glioblastoma Multiforme  [pubmed:28667042]
Archives of Gynecology and Obstetrics	Is there any relationship between adipocytokines and angiogenesis factors to address endothelial dysfunction and platelet aggregation in untreated patients with preeclampsia? [10.1007/s00404-017-4461-2]
EXPERIMENTAL AND THERAPEUTIC MEDICINE	Expression of hypoxia-inducible factor-1α in synovial fluid and articular cartilage is associated with disease severity in knee [pubmed:28123469]
Medical Journal Armed Forces India	Study of acute hypoxia markers in healthy subjects: Utility in post-crash investigation [10.1016/j.mjafi.2017.04.003]