血管内皮生长因子A(VEGFA)检测试剂盒(酶联免疫吸附试验

血管内皮生长因子A(VEGFA)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Vascular Endothelial Growth Factor A (VEGFA)

VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor

优尔生一级代理：杭州昊鑫生物
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优尔生位于武汉出口加工区，已整体通过ISO 9001:2008、ISO 13485:2003质量管理体系认证，以提供生命科学科研用检测试剂为主，75%以上的服务对象位于欧美国家。凭借着深厚的技术积累和庞大的引物、cDNA、质粒、杂交瘤细胞“种子库”和检测试剂半成品库库存，能够做到约11,000种蛋白、19,000种抗体以及7,000种检测试剂盒立等可取。蛋白项目部拥有四大技术平台，包括多肽合成平台、小分子抗原性改造平台、天然蛋白提取平台和采用原核（E.Coli）表达系统与真核（酵母、杆状病毒-昆虫细胞、哺乳动物细胞）表达系统的重组蛋白表达平台。抗体项目部拥有完备的单克隆抗体和多克隆抗体制备平台。免疫应用部负责检测试剂盒的研制，主要是ELISA和CLIA试剂盒。成品检测部负责所有蛋白、抗体以及检测试剂盒产成品的检测及质量控制。公司实行三级质量控制，包括原料检测、半成品检测及成品检测。
 

• 编号SEA143Ga
• 物种Chicken (Gallus，鸡) 相同的名称，不同的物种。
• 实验方法双抗夹心
• 反应时长3h
• 检测范围15.6-1,000pg/mL
• 灵敏度最小可检测剂量小于等于6.3pg/mL.
• 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• 下载英文说明书   中文说明书
• 规格48T96T 96T*5 96T*10 96T*100
• 价格¥ 2873

产品包装图模拟

特异性

本试剂盒用于检测血管内皮生长因子A(VEGFA)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的血管内皮生长因子A(VEGFA)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本	回收率范围(%)	平均回收率(%)
serum(n=5)	79-99	91
EDTA plasma(n=5)	97-105	101
heparin plasma(n=5)	80-96	86

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100 
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10% 
批间差： CV<12% 

线性

在定值血清及血浆样本内加入适量的血管内皮生长因子A(VEGFA)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中血管内皮生长因子A(VEGFA)含量的测定值与理论值的比率。

样本	1:2	1:4	1:8	1:16
serum(n=5)	97-105%	88-102%	82-91%	81-105%
EDTA plasma(n=5)	96-103%	78-101%	99-105%	78-101%
heparin plasma(n=5)	93-105%	78-97%	93-101%	78-104%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将血管内皮生长因子A(VEGFA)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的血管内皮生长因子A(VEGFA)与连接于固相载体上的抗体结合，然后加入生物素化的血管内皮生长因子A(VEGFA)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血管内皮生长因子A(VEGFA)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

参考文献

杂志	参考文献
The Journal of Biological Chemistry	Intertissue flow of GSH as a tumor growth promoting mechanism: interleukin 6 induces GSH release from hepatocytes in metastatic B16 melanoma-bearing mice [Jcb: 196261]
Turkish Journal of Veterinary and Animal Sciences	Can the vascular endothelial growth factor (VEGF) and total estradiol 17β be used as a marker of equine oocyte maturation? [Tubitak: source]
Regulatory Peptides	The correlation between resting serum leptin and serum angiogenic indices at rest and after submaximal exercise [ScienceDirect: S016701151100156X]
Acta Biomaterialia	Multiple release of polyplexes of plasmids VEGF and bFGF from electrospun fibrous scaffolds towards regeneration of mature blood vessels [PubMed: 22484697]
Oncogene	Elevated PLGF contributes to small-cell lung cancer brain metastasis [PubMed: 22797069]
International Journal of Nephrology	Impairment and Differential Expression of PR3 and MPO on Peripheral Myelomonocytic Cells with Endothelial Properties in Granulomatosis with Polyangiitis [PubMed: 22792461]
Zahedan Journal of Research in Medical Sciences	The Effect of Acute Sub-Maximal Endurance Exercise on Serum Angiogenic Indices in Sedentary Men [Zjrms: Source]
Nanoscale Research Letters	Silver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart. [PubMed: 22827927]
BMC cell biology	BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause [PubMed: PMC3640998]
PLoS One.	Molecular Effects of Doxycycline Treatment on Pterygium as Revealed by Massive Transcriptome Sequencing [PubMed: PMC3378547]
Oncology Reports	A bone-seeking clone exhibits different biological properties from the ACHN parental human renal cell carcinoma in vivo and in vitro [PubMed: PMC3583590]
The Journal of Biological Chemistry	Intertissue Flow of Glutathione (GSH) as a Tumor Growth-promoting Mechanism: INTERLEUKIN 6 INDUCES GSH RELEASE FROM HEPATOCYTES IN METASTATIC B16 MELANOMA-BEARING MICE [PubMed: PMC3091180]
International Journal of Molecular Sciences	Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral Muscles [NCBI:PMC3856104]
Archives of Animal Nutrition	Nano-nutrition of chicken embryos. The effect of silver nanoparticles and ATP on expression of chosen genes involved in myogenesis [Pubmed: 23952606]
Angiogenesis	Prodrug of green tea epigallocatechin-3-gallate (Pro-EGCG) as a potent anti-angiogenesis agent for endometriosis in mice [Pubmed: 22948799]
Experimental Eye Research	Hyperglycemia promotes vasculogenesis in choroidal neovascularization in diabetic mice by stimulating VEGF and SDF-1 expression in retinal pigment epithelial cells [Pubmed: 24780853]
Tumor Biology	Biglycan up-regulated vascular endothelial growth factor (VEGF) expression and promoted angiogenesis in colon cancer [Pubmed:25371074]
Archives of Animal Nutrition	Effect of silver nanoparticles and hydroxyproline, administered in ovo, on the development of blood vessels and cartilage collagen structure in chicken embryos [Pubmed:25530495]
Stem Cells International	Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro [Pubmed:25767513]
Physiology and Pharmacology	A Study on the Serum Levels of Angiogenic Factors in Response to Acute Long-term Submaximal Exercise in Sedentary Men [Phypha:Source]
Int J Clin Exp Med.	The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell [PubMed: 26628972]
Mol Med Rep	Application of recombinant peroxisome proliferator [PubMed: 26676891]