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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期:
12个月
- 英文名:
Recombinant Human Glutathione S-transferase kappa 1 (GSTK1), partial (Active)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
10ug/20ug/50ug/100ug/200ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥69.0 |
|---|---|---|---|
| 规格: | 20ug | 产品价格: | ¥139.0 |
| 规格: | 50ug | 产品价格: | ¥209.0 |
| 规格: | 100ug | 产品价格: | ¥349.0 |
| 规格: | 200ug | 产品价格: | ¥419.0 |
| 规格: | 500ug | 产品价格: | ¥559.0 |
| 规格: | 1mg | 产品价格: | ¥699.0 |
Research Areas
Tags & Cell MarkersUniprot ID
Q9Y2Q3Gene Names
GSTK1Alternative Name(s)
GST 13-13 GST class-kappa GSTK1-1Lead Time
3-7 business daysOrganism
Homo sapiens (Human)Source
E.coliExpression Region
2-226aaProtein Length
PartialTag Info
N-terminal GST-taggedTarget Protein Sequence
GPLPRTVELFYDVLSPYSWLGFEILCRYQNIWNINLQLRPSLITGIMKDSGNKPPGLLPRKGLYMANDLKLLRHHLQIPIHFPKDFLSVMLEKGSLSAMRFLTAVNLEHPEMLEKASRELWMRVWSRNEDITEPQSILAAAEKAGMSAEQAQGLLEKIATPKVKNQLKETTEAACRYGAFGLPITVAHVDGQTHMLFGSDRMELLAHLLGEKWMGPIPPAVNARLMW
52.4 kDaPurity
Greater than 90% as determined by SDS-PAGE.Form
Lyophilized powder.If the size you ordered does not have lyophilized powder in stock, we will provide it in liquid formBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验GST fusion protein purification--GST融合蛋白纯化
GST fusion protein purification GST融合蛋白纯化John Mundy,Institute of Molecular Biology,Copenhagen,Denmark1)grow 20ml cells O/N37℃ dilute 50X into prewarmed LB,grow to 0.6 OD or about1hr.Induce w/ 2mM IPTG (238mg/0.5l),grow 3hr,spin 6k GS3 10',freeze
原理 GST 纯化系统是利用GST (glutathione-S-transferase )融合蛋白与固定的谷胱甘肽(GSH)通过硫键共价亲和,通过GSH交换洗脱的原理来进行纯化 。1ml树脂大约可结合5-8 mg融合蛋白,并可反复使用数次。试剂u IPTG(异丙基硫代-β-D-半乳糖苷) 2g IPTG 溶解入10ml 水,过滤除菌,分装,-20℃保存。u Lysis buffer (50ml)1)2.5ml 1 M Tris,pH8.0 2)0.1ml 0.5ml EDTA 3)0.292
相关专题 蛋白表达技术全攻略 本指南介绍了大规模和小规模GST蛋白表达 和纯化的方法。由于蛋白的多样性,因此推荐先进小规模表达及纯化测试。 材料与试剂 1. IPTG (Sigma) 2. 咪唑 (Sigma) 3. 蛋白酶抑制剂 (Roche) 4. 溶菌酶(Sigma) 5. 谷胱甘肽 琼脂 糖 4B 珠 (Pierce 15160) 6. 谷胱
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