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- 详细信息
- 技术资料
- 保存条件:
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
- 保质期:
详见说明
- 英文名:
Recombinant Endothelial Cell Specific Molecule 1 (ESM1)
- 库存:
充足
- 供应商:
cloud-clone corp(优尔)
- 规格:
10ug
内皮细胞特异分子1(ESM1)重组蛋白
Purity≥ 92%;Fragment:Ala22~Arg184; Predicted Molecular Mass(KD):19.3kDa优尔生的一级代理:杭州昊鑫生物
优尔生位于武汉出口加工区,已整体通过ISO 9001:2008、ISO 13485:2003质量管理体系认证,以提供生命科学科研用检测试剂为主,75%以上的服务对象位于欧美国家。凭借着深厚的技术积累和庞大的引物、cDNA、质粒、杂交瘤细胞“种子库”和检测试剂半成品库库存,能够做到约11,000种蛋白、19,000种抗体以及74986种检测试剂盒立等可取。蛋白项目部拥有四大技术平台,包括多肽合成平台、小分子抗原性改造平台、天然蛋白提取平台和采用原核(E.Coli)表达系统与真母、杆状病毒-昆虫细胞、哺乳动物细胞)表达系统的重组蛋白表达平台。抗体项目部拥有完备的单克隆抗体和多克隆抗体制备平台。免疫应用部负责检测试剂盒的研制,主要是ELISA和CLIA试剂盒。成品检测部负责所有蛋白、抗体以及检测试剂盒产成品的检测及质量控制。公司实行三级质量控制,包括原料检测、半成品检测及成品检测。
- 编号RPC463Ra01
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 来源原核表达
- 宿主E.coli
- 内毒素水平<1.0EU/µg(LAL法测定)
- 亚细胞定位分泌
- 预测分子量19.3kDa
- 实际分子量20kDa(差异分析请参阅说明书)
- 片段与标签Ala22~Arg184 with N-terminal His Tag
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
- 性状冻干粉
- 纯度> 90%
- 等电点5.7
- 应用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白,请参见活性蛋白。 - 下载英文说明书 中文说明书
- 规格10µg50µg 200µg 1mg 5mg
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
参考文献
| 杂志 | 参考文献 |
| Urology | Detection on dynamic changes of endothelial cell specific molecule-1 in acute rejection after renal transplantation [PubMed: 22608803] |
| Microvasc Res. | Endocan is useful biomarker of survival and severity in sepsis. [Pubmed: 24769132] |
| J Matern Fetal Neonatal Med. | Endocan, a putative endothelial cell marker, is elevated in preeclampsia, decreased in acute pyelonephritis, and unchanged in other obstetrical syndromes [Pubmed:25211383] |
| Microvasc Res. | Endocan is useful biomarker of survival and severity in sepsis [Pubmed:24769132] |
| Angiology | Endocan Levels and Subclinical Atherosclerosis in Patients With Systemic Lupus Erythematosus [PubMed: 26614790] |
| Archives of Medical Science - Atherosclerotic Diseases | Assessment of subclinical cardiac damage in chronic plaque psoriasis patients: a case control study [Journal:-102] |
| Int Immunopharmacol. | Inhibition of endocan attenuates monocrotaline-induced connective tissue disease related pulmonary arterial hypertension. [pubmed:27912147] |
| Oncotarget | Diagnostic and prognostic values of endothelial-cell-specific molecule-1 with malignant pleural effusions in patients with non-small cell lung cancer [pubmed:28514746] |
| Biomedical and Pharmacology Journal | Changes in Endocan Levels and Blood Coagulation in HIV Infection [DOI: 10.13005/bpj/1082] |
| Life Sciences | A novel role of endocan in alleviating LPS-induced acute lung injury [Pubmed:29627442] |
| International Urology and Nephrology | Is the plasma endocan level a reliable predictor for the severity of erectile dysfunction? [Pubmed:30054863] |
| The Open Rheumatology Journal | Serum Level of Endothelial Cell-Specific Molecule-1 (ESM-1) as a New Potential Biomarker for Rheumatoid Arthritis Disease Activity [Doi: 10.2174/1874312901812010189] |
| Molecular Medicine Reports | Downregulation of ENDOCAN in myeloid leukemia cells inhibits proliferation and promotes apoptosis by suppressing nuclear factor‑κB activity [Pubmed: 30816462] |
| Biomarkers in Medicine | Endothelial biomarkers in the light of new sepsis definition [Pubmed: 30920847] |
| Experimental and Therapeutic Medicine | Risk stratification and prognostic evaluation of endothelial cell‑specific molecule1, von Willebrand factor, and a disintegrin‑like and metalloprotease with … [] |
| Turkish Journal of Medical Sciences | A novel marker relationship between carotid intima–media thickness and disease activity score-28 in patients with rheumatoid arthritis: human endothelial cell … [Pubmed: 31655533] |
| Biomarkers | Role of Ascitic Endocan Levels in the Diagnosis of Spontaneous Bacterial Peritonitis in Decompensated Cirrhosis [Pubmed: 32364003] |
| J Obstet Gynaecol Res | Comparison of the maternal serum endocan levels in preterm premature rupture of membrane and normal pregnancy [34109715] |
| turkish journal clinics laboratory | The relationship between serum endocan levels with the presence of contrast-induced nephropathy in patients undergoing coronary angiography [] |
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内皮细胞特异分子1(ESM1)重组蛋白
¥931







