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- 供应商:
上海古朵生物科技有限公司
- 规格:
100 mg
PackSize:100 mg
ProductName:tRNA, from Baker's Yeast, 100
供应商:上海古朵生物科技公司
Roche
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文献和实验【转帖】Top 100 proteomics publications
Top 100 proteomics publications ----From Ion Channel Media Group:ttp://www.ionchannelmedia.com/websites.php 1. Gavin et al. (2006) Proteome survey reveals modularity of the yeast cell machinery. Nature 2. Bonetta et al. (2006) Protein
(determine experimentally). A dilution series of 1:100 to 1:10,000 should cover it. Incubate at RT for 1 hr or longer, depending on the antibody. Sometimes an overnight incubation is helpful. Jon''s anti-actin Guinea pig antibodies from animal#1 are best
Alternative: Instead of recombinant lyticase, can also use Zymolyase 100T from ICN. Use ~2 mg per 2 liters of cells. The amount required can vary from 2-10 mg depending on the yeast strain. If using zymolyase, add in one extra YPD/S washing step
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