丝裂霉素 C 来源于头状链霉菌，Mitomycin C fr
om Streptomyces caespitosus powder, contains NaCl as solubilizer- ¥1150
- sigma
- 美国
- M0503
- 2025年10月01日
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- 询价记录
- 文献和实验
- 技术资料
- 英文名：
Mitomycin C from Streptomyces caespitosus powder, contains NaCl as solubilizer
- 库存：
大量
- 供应商：
上海觅拓
- CAS号：
50-07-7
- 规格：
2MG
Application
Mitomycin C is produced by a strain of actinomyces, Streptomyces caespitosus. It contains three anticancer moieties, quinine, urethane, and aziridine groups. It is used to generate mitotically inactive feeder cells in cell culture systems, such as the mitotically inactive fibroblasts used in embryonic stem cell systems.
Biochem/physiol Actions
DNA 合成、细胞核分裂和癌细胞的抑制剂。对革兰氏阳性菌、
Caution
This vial contains 2 mg Mitomycin C and 48 mg NaCl. Stock solutions should be filter sterilized and stored at 2-8°C in the dark. Solutions at pH 6-9 can be stored at 0-5°C for up to a week, but if a precipitate forms, a fresh solution should be prepared - the precipitated solution has been proven toxic to cells.
Preparation Note
Mitomycin C is soluble in water at .5 mg/mL, with a pH of 6.0-7.5. It undergoes rapid degradation in acidic solutions with pH<6, and is mostly likely to retain activity in solutions with a pH between 6-9.
Protocols & Applications
性质
| Related Categories | Antibiotics, Antibiotics A to Z, Antibiotics G-M,Antibiotics by Application, Antineoplastic and Immunosuppressive Antibiotics, |
| form | powder |
| contains | NaCl as solubilizer |
| solubility | H2O: soluble1 vial + 4 mL, clear to slightly hazy, blue to purple |
| Mode of action | DNA synthesis | interferes |
| antibiotic activity spectrum | Gram-negative bacteria |
| Gram-positive bacteria | |
| storage temp. | 2-8°C |
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C15H18N4O5
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文献和实验Preabsorbing Antisera with C. elegans Acetone Powder
Preabsorbing Antisera with C. elegans Acetone Powder by Michael Koelle, adapted from "Antibodies: A Practical Approach" by D. Catty One way to eliminate crossreactivity of a polyclonal antiserum raised against a worm
Streptomyces:Protocols/Conjugation
-NaCl, SFM+MgCl2 , 2xYT, sterile distilled water (sdH2 O), 15mL falcon tube(s), micro-centrifuge tube(s) (MCT), heating block @ 50°C, incubator @ 30°C, falcon centrifuge, micro-centrifuge, vortexer, Antibiotic(s). Preparation Pour 4 plates
Streptomyces:Protocols/Mini-Maxi Prep
@ ~4°C, micro-centrifuge @ ~4°C. Optional: incubator (i.e. a water bath) @ 37°C, Ribonuclease A (RNase). Preparation Chill Solution I and Solution III on ice; the isopropanol and 70% ethanol should be placed in the freezer. Make fresh Solution
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