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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温保存
- 库存:
现货
- 供应商:
鹿森生物
- CAS号:
67-42-5
- 规格:
瓶装
应用
乙二醇双(2-氨基乙*)四乙酸(EGTA)可用于:
作为大鼠主动脉弓缩窄术(TAC)用低温心脏停跳液的组分
作为冰冷裂解缓冲液组分,用于蛋白提取和全细胞提取
作为HEK293F细胞线粒体提取用缓冲液A、B和C的组分乙*
一种螯合剂,可在镁存在的情况下对钙进行测定。
生化/生理作用
乙二醇双(2-氨基乙*)四乙酸(EGTA)A是一种螯合剂,可在镁存在的情况下对钙进行测定。
物理属性
在水中的溶解性随 pH 值增加而增加
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文献和实验Transduction without tip links in cochlear hair cells is mediated by ion channels with permeation properties distinct from those of the mechano-electrical transducer channel.
Walter Marcotti et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(16), 5505-5514 (2014-04-18)
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of aggregation plate Materials: Dissecting microscope; Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3); 1 ml syringe with 26 G 1/2 needle of KSOM medium; light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);
of mutants need to be made. 1) Design oligo - the forward and reverse primer can be bought ready-made (but may be cheaper to synthesise it), they prime to regions in front of and behind the cloning region (e.g. T7 promoter primer and T7 terminator primer
b-Gal Staining of Murine Embryos
) (Sigma E-4378) Wash buffer : 200ml of wash buffer combine by 23ml of 0.2M NaH2PO4.H2O, 77ml of 0.2M Na2HPO4, 400ml of 1M MgCl2 2ml of 0.5M EGTA 0.2ml of 20% NP-40 0.2ml of 10% Na Deoxycholate DDW up to 200ml Fixing buffer : 198ml 0.1M
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