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- 技术资料
- 保存条件:
2-8℃
- 英文名:
LectinfromTriticumvulgaris
- 库存:
33
- 供应商:
莼试
产品名称:麦胚凝集素
英文名:LectinfromTriticumvulgaris
别名:
介绍:TriticumvulgarislectinorWeatgermagglutinin(WGA)isisolatedfromTriticumvulgaris(Weatgerm)andpurifiedbyaffinitychromatography.Thelectinhastwosubunitsandamolecularweightof36kDa.WGAselectivelybindstoN-Acetylglucosamine(GlcNAc)andtoN-acetylneuraminicacid(sialicacid)residuesofglycoproteinsandglycolipids.TheWGAlectinagglutinateserythrocytesandmosttypesofmalignantcells.Itagglutinatesrabbiterythrocytesat<0.1μg/mlfollowingtrypsintreatmentofthecells.Adding300mMN-Acetyle-D-Glucosaminegivesaninhibitionwithatiterthatisatleast8-foldlowerthanthecontrol.WheatgermagglutinininhibitstheC5areceptorinteraction,whichhasimplicationsinstudiesofreceptormicro-heterogeneityandligandbindingsites.WGAtogetherwithConAisthelectinmostwidelyusedasananalyticalandpreparativeagentwhenstudyingglycoproteinsandcellsurfaceproteins.Theimmobilizedlectincanbeusedforaffinitychromatographyofcellsandsub-cellularparticles.WGAissuppliedasawhitetopaleyellowlyophilizedpowderfromabuffercontaining10mMCH3COONH4.Nopreservativesareadded.ThepurityofWGAisdeterminedbySDS-electrophoresiswhichgeneratesthreebands,correspondingtothethreeisolectinsofWGA.Proteincontentisverifiedinspectrophotmetry.
储存条件:2-8℃
英文名:LectinfromTriticumvulgaris
别名:
介绍:TriticumvulgarislectinorWeatgermagglutinin(WGA)isisolatedfromTriticumvulgaris(Weatgerm)andpurifiedbyaffinitychromatography.Thelectinhastwosubunitsandamolecularweightof36kDa.WGAselectivelybindstoN-Acetylglucosamine(GlcNAc)andtoN-acetylneuraminicacid(sialicacid)residuesofglycoproteinsandglycolipids.TheWGAlectinagglutinateserythrocytesandmosttypesofmalignantcells.Itagglutinatesrabbiterythrocytesat<0.1μg/mlfollowingtrypsintreatmentofthecells.Adding300mMN-Acetyle-D-Glucosaminegivesaninhibitionwithatiterthatisatleast8-foldlowerthanthecontrol.WheatgermagglutinininhibitstheC5areceptorinteraction,whichhasimplicationsinstudiesofreceptormicro-heterogeneityandligandbindingsites.WGAtogetherwithConAisthelectinmostwidelyusedasananalyticalandpreparativeagentwhenstudyingglycoproteinsandcellsurfaceproteins.Theimmobilizedlectincanbeusedforaffinitychromatographyofcellsandsub-cellularparticles.WGAissuppliedasawhitetopaleyellowlyophilizedpowderfromabuffercontaining10mMCH3COONH4.Nopreservativesareadded.ThepurityofWGAisdeterminedbySDS-electrophoresiswhichgeneratesthreebands,correspondingtothethreeisolectinsofWGA.Proteincontentisverifiedinspectrophotmetry.
储存条件:2-8℃
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麦胚凝集素
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