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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名:
HPAEpiC
- 库存:
现货库存
- 供应商:
华拓生物原代细胞
- 肿瘤类型:
见细胞说明
- 细胞类型:
科研细胞
- 品系:
HPAEpiC人肺泡上皮细胞
- 组织来源:
肺泡上皮细胞
- 相关疾病:
请联系我们发送详细说明
- 物种来源:
人肺泡上皮细胞
- 免疫类型:
见细胞资料
- 细胞形态:
请联系我们发送详细资料
- 是否是肿瘤细胞:
见细胞类型信息
- 器官来源:
人
- 运输方式:
常温或冻存发货,空运快递
- 年限:
目前代数:三代内
- 生长状态:
贴壁
- 规格:
2X10^6 cells
| 名称: | HPAEpiC人肺泡上皮细胞 |
| 细胞描述: | 肺泡;上皮细胞 |
| 货号: | HTX2419 |
| 培养基: | 89%DMEM+10%FBS+1%双抗 |
| 生长状态: | 贴壁生长 |
| 背景介绍: | HPAEpiC人肺泡上皮细胞,肺中的支气管经多次反复分枝成无数细支气管,它们的末端膨大成囊,囊的四周有很多突出的小囊泡,即为肺泡。 |
| 检测: | HPAEpiC人肺泡上皮细胞HPAEpiC存种活性检测;标志物鉴定;衣原体、支原体检测(HPAEpiC人肺泡上皮细胞) |
| 背景描述: | HPAEpiC人肺泡上皮细胞,分Ⅰ型肺泡细胞和Ⅱ型肺泡细胞小肺泡细胞。 |
1. Tingting Lv, et al. Can Respir J. 2024; 2024: 9284430. PMID: 39139502
IL-17 Mildly Rescued the Impaired Proliferation of Alveolar Epithelial Cells Induced by LCN2 Overexpression
2. Ting‐Ting Chen, et al. Adv Sci (Weinh). 2025 Mar 17;12(18):2414211. PMID: 40091520
ADAR1‐HNRNPL‐Mediated CircCANX Decline Promotes Autophagy in Chronic Obstructive Pulmonary Disease


The bronchi in the lungs are repeatedly branched into numerous bronchioles, their ends bulging into sacs surrounded by many small protruding vesicles called alveoli. Small alveolar cells, also known as type I alveolar cells, are about 0.1 microns thick and have a basement membrane at the base. Large alveolar cells, also known as type II alveolar cells, secrete a surface active substance (dipalmitoyl lecithin) to reduce alveolar surface tension. Type II alveolar cells were located between type I alveolar cells, and the number of type II cells was more than that of type I alveolar cells, but the coverage area was smaller than that of type I alveolar cells. The cells are cuboidal or round, with their tips protruding into the alveolar cavity. The nucleus is round and the cytoplasm is light colored and foamy. Under electron microscope, the cells were free and had a few microvilli, rich mitochondria and lysosomes in the cytoplasm, and well developed rough endoplasmic reticulum and Golgi complex. There are more secretory particles above the nucleus, with high electron density and varying sizes. The particles with a diameter of 0.1-1.0μm contain parallel lamellar structures, which are called hungry lamellar bodies. The main components in the body are phospholipids, mainly dipalmitoyl lecithin, in addition to glycosaminoglycan and protein. After the particles are released, a layer of mucus, called surfactant, forms on the surface of the alveoli. Surfactant can reduce the surface tension of alveoli and stabilize the size of alveoli. During exhalation, alveolar shrinkage, surface active substance density increase, surface tension decrease, prevent excessive collapse of alveolar; During inhalation, the alveolar dilates, the density of surface active substances decreases, and the alveolar retraction force increases, which can prevent excessive alveolar expansion.
深圳市豪地华拓生物科技有限公司(www.otwobiotech.com)
HPAEpiC人肺泡上皮细胞技术咨询方式

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文献和实验查看HPAEpiC细胞文献:www.otwobiotech.com
问:我打算检测线粒体膜电位,大鼠肺移植后,如何分离肺泡上皮细胞呢?答:步骤如下:1、取得完全无血液残留的肺脏:实验前将大鼠全身血液放净,取得完全无血液残留的肺脏。2、消化肺组织:常用于细胞消化的酶有胰蛋白酶、弹性蛋白酶、胶原酶。胰蛋白酶是最早用于消化、分离上皮细胞的酶,现在一般用损伤性小的弹性蛋白酶来替代胰蛋白酶。但这种酶却有活性不稳定,有效作用时间短暂,容易自我降解等问题。Dobbs L G等认为弹性蛋白酶能更有选择性的消化分离上皮细胞[9]。胰蛋白酶消化没有选择性,会带来像内皮细胞和间质细胞
一、取得完全无血液残留的肺脏: 实验前j将大鼠全身血液放净,取得完全无血液残留的肺脏二、消化肺组织:常用于细胞消化的酶有胰蛋白酶、弹性蛋白酶、胶原酶。胰蛋白酶是最早用于消化、分离上皮细胞的酶,现在一般用损伤性小的弹性蛋白酶来替代胰蛋白酶。但这种酶却有活性不稳定,有效作用时间短暂,容易自我降解等问题。Dobbs L G等认为弹性蛋白酶能更有选择性的消化分离上皮细胞[9]。胰蛋白酶消化没有选择性,会带来像内皮细胞和间质细胞等一些不能在后面的分离步骤中去除的杂质细胞,因此弹性蛋白酶更有助于提高上皮细胞
原代 肺泡上皮 细 胞 ( Primary Alveolar Epithelial Cell s ) 的体外分离培养 1 、完全无血液残留肺 脏组织 2、消化肺 组 织 :常用细胞消化酶:胰蛋白酶、弹性蛋白酶、胶原酶。经支气管将酶灌注到完整的肺中消化,或把肺组织剪碎放入消化液中消化。前者比后者更有效。3 、分离 纯 化 细 胞 :原代肺泡上皮细胞的分离纯化主要方法:(1)密度梯度离心法:密度梯度离心分离细胞的原理是不同细胞的沉降系数不同,在密度梯度离心中细胞所处的位置也相应不同
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