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文献和实验Hydrogen Peroxide Assay for Amine Oxidase Activity
Amine oxidase activity can be assessed by measuring the conversion of substrate to product, or by measuring the formation of concomitantly formed hydrogen peroxide. Fluorometry offers greater sensitivity and selectivity than conventional
Measurement of Aldehyde Formation as an Assay for Polyamine Oxidase Activity
The polyamines spermine and spermidine can be metabolized by polyamine oxidases in two ways, oxidative determination or oxidative cleavage ( 1 , 2 ) ( see Chapter 1 ). An example of the former is the amine oxidase present in bovine
Radiochemical Assay of Diamine Oxidase
Diamine oxidase activity (E.C. 1.4.3.6) can be measured by a modification of the method first reported by Okuyama and Kobayashi ( 1 ), as developed by Trydingetal. ( 2 – 4 ). Oxidation of [1,4- 14 C]putrescine by diamine oxidase leads
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