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- 2025年07月15日
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100 assays
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文献和实验media (= RPMI 1640 + 10% FBS) PBS + 2% EDTA Triton X-100 solution (10% in dH2 O) Effector cell preparation: The mouse NK-cell line KY1 is used as an effector cell. During recovery (e.g. after freezing), these cells require 1000 U
killing (e.g. antibody-mediated cytotoxicity). After the incubation, add 20 µl 10% Triton X-100 to each positive control. Pipet up and down to achieve complete lysis. Spin 96-well plates at 500 g for 5 minutes. Carefully pipet 100
Mouse Embryonic Stem Cell Adherent Cell Differentiation and Cytotoxicity Assay
their potential developmental hazard. An adherent cell differentiation and cytotoxicity (ACDC) assay was developed using pluripotent mouse embryonic stem cells (mESCs) to evaluate chemical-induced effects on both stem cell viability and differentiation. This assay
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